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Team:Rice University/Notebook/12 June 2008
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=Thursday 12 June= | =Thursday 12 June= | ||
*Taylor Stevenson | *Taylor Stevenson | ||
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**Transformation From New Registry (Paper Punches)-[[Team:Rice University/Electroporation|Electroporation]] and [[Team:Rice_University/Heat_Shock|Heat Shock]] of [http://partsregistry.org/Part:BBa_I744204 BBA_I744204] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] (positive control) and [http://partsregistry.org/Part:BBa_I13521 BBA_I13521] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into XL1-Blue MR electrocompetent and XL1-Blue chemically competent cells. | **Transformation From New Registry (Paper Punches)-[[Team:Rice University/Electroporation|Electroporation]] and [[Team:Rice_University/Heat_Shock|Heat Shock]] of [http://partsregistry.org/Part:BBa_I744204 BBA_I744204] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] (positive control) and [http://partsregistry.org/Part:BBa_I13521 BBA_I13521] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into XL1-Blue MR electrocompetent and XL1-Blue chemically competent cells. | ||
| - | *#Added 1uL of stock miniprepped [http://partsregistry.org/Part:BBa_I744204 BBA_I744204] DNA solution and 2uL obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 50*C for 30min to both chemical and electrocompetent cells. | + | |
| + | *#Added 1uL of stock miniprepped [http://partsregistry.org/Part:BBa_I744204 BBA_I744204] DNA solution and 2uL obtained by soaking [http://partsregistry.org/Part:BBa_I13521 BBA_I13521] DNA punch in (10:1) Tris:EDTA buffer incubated @ 50*C for 30min to both chemical and electrocompetent cells. | ||
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*#Electropulse parameters for the miniprepped plasmid and extracted plasmid where 1.8kV & 5.2ms and 1.8kV & 5.0ms respectively. | *#Electropulse parameters for the miniprepped plasmid and extracted plasmid where 1.8kV & 5.2ms and 1.8kV & 5.0ms respectively. | ||
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*#Plated 100uL of each the transformed and cultured cells on LB/Amp plates. Plates were cultured O/N @ 37*C. | *#Plated 100uL of each the transformed and cultured cells on LB/Amp plates. Plates were cultured O/N @ 37*C. | ||
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**'''Result'''-no colonies were observed on plates from the extracted DNA while >>1,000 colonies grew on plates resulting from miniprepped DNA. | **'''Result'''-no colonies were observed on plates from the extracted DNA while >>1,000 colonies grew on plates resulting from miniprepped DNA. | ||
Revision as of 17:32, 25 June 2008
Thursday 12 June
- Taylor Stevenson
- Transformation From New Registry (Paper Punches)-Electroporation and Heat Shock of [http://partsregistry.org/Part:BBa_I744204 BBA_I744204] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] (positive control) and [http://partsregistry.org/Part:BBa_I13521 BBA_I13521] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into XL1-Blue MR electrocompetent and XL1-Blue chemically competent cells.
- Added 1uL of stock miniprepped [http://partsregistry.org/Part:BBa_I744204 BBA_I744204] DNA solution and 2uL obtained by soaking [http://partsregistry.org/Part:BBa_I13521 BBA_I13521] DNA punch in (10:1) Tris:EDTA buffer incubated @ 50*C for 30min to both chemical and electrocompetent cells.
- Electropulse parameters for the miniprepped plasmid and extracted plasmid where 1.8kV & 5.2ms and 1.8kV & 5.0ms respectively.
- Plated 100uL of each the transformed and cultured cells on LB/Amp plates. Plates were cultured O/N @ 37*C.
- Result-no colonies were observed on plates from the extracted DNA while >>1,000 colonies grew on plates resulting from miniprepped DNA.
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