Latest revision as of 19:15, 25 June 2008

Saturday 7 June

Selim Sheikh:
- Prepared digestion of lambda DNA with XhoI only
- Prepared gel electrophoresis of above digest along with solution of lambda DNA with 1Kb standard

(from right to left, lane 1: 1Kb, lane 2: lambda DNA, lane 3: lambda with XhoI)

Taylor Stevenson
- Phage infected XL1-blue MR colonies cultured in 96 deep-well plate were spotted onto two EMB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).
- Result-both plates showed no bacterial growth after 24h.

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

@@ Line 4: / Line 4: @@
 **Prepared gel electrophoresis of above digest along with solution of lambda DNA with 1Kb standard
 [[Image:20080607_gel.jpg|100px|left]]
-(from left to right, lane 1: 1Kb, lane 2: lambda DNA, lane 3: lambda with XhoI)
+(from right to left, lane 1: 1Kb, lane 2: lambda DNA, lane 3: lambda with XhoI)
 <BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR>
 *Taylor Stevenson