Project
From 2008.igem.org
ZhangHaoran (Talk | contribs) |
|||
Line 67: | Line 67: | ||
.STYLE30 {color: #B9E5ED} | .STYLE30 {color: #B9E5ED} | ||
.STYLE31 {font-size: 30px} | .STYLE31 {font-size: 30px} | ||
+ | .STYLE36 {color: #0000FF} | ||
--> | --> | ||
</style> | </style> | ||
Line 149: | Line 150: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td colspan="2" bgcolor="#0000FF"><span class=" | + | <td colspan="2" bgcolor="#0000FF"><span class="STYLE36">a</span></td> |
</tr> | </tr> | ||
</table> | </table> | ||
Line 179: | Line 180: | ||
<p>The second one is the Helper section. We call it helper section because the LuxR protein is the prerequisite for the activation of PLux. Here we used a constitutive promoter to regulate the expression of the LuxR protein. </p> | <p>The second one is the Helper section. We call it helper section because the LuxR protein is the prerequisite for the activation of PLux. Here we used a constitutive promoter to regulate the expression of the LuxR protein. </p> | ||
<p>The core section is the convertible switch. Convertible switch is a genetic device that can switch between two convertible states, which, here, represents a different survival strategy for the cells each. </p> | <p>The core section is the convertible switch. Convertible switch is a genetic device that can switch between two convertible states, which, here, represents a different survival strategy for the cells each. </p> | ||
- | <p>When adding | + | <p>When adding Arabinose/AHL different genes will get expressed behind the two mutually-repressive promoters. That means when added into the culture, AHL will diffuse into the cell bind the LuxR protein and form a complex which can activate the LuxPr promoter and then the genes of rhII capR and araC will express. Then the AraC protein will bind to the PBad/araC promoter and repress the expression of the aiiA and another capR gene. However, you can turn the switch to the other side by adding Arobinose. When adding Arobinose into the culture, the repression functional molecular AraC protein will get released from the PBad/AraC promoter. With the expression of the aiiA gene the signal molecular will get digested and therefore decrease to a proper level which is not high enough to activate the LuxPr promoter.<br> |
The most important thing in this section is the capacity of the two different promoters luxPr and PBad/araC are quite different. When the luxRr promoter is activated, its higher capacity will express more chloromycetin resistant protein and another important thing is by sensing the AHL which is sent out by cell 2 it can produce another kind of signal molecular BHL.<br> | The most important thing in this section is the capacity of the two different promoters luxPr and PBad/araC are quite different. When the luxRr promoter is activated, its higher capacity will express more chloromycetin resistant protein and another important thing is by sensing the AHL which is sent out by cell 2 it can produce another kind of signal molecular BHL.<br> | ||
Cell 2 is similarly designed as Cell 1</p> | Cell 2 is similarly designed as Cell 1</p> | ||
Line 227: | Line 228: | ||
<td colspan="2" bgcolor="#03438A"><table width="980" border="0"> | <td colspan="2" bgcolor="#03438A"><table width="980" border="0"> | ||
<tr> | <tr> | ||
- | <td width="477"><div align="center"><span class="STYLE17"><a href="https://2008.igem.org/Experiment">Experiment</a></span> </div></td> | + | <td width="477" bgcolor="#03438A"><div align="center"><span class="STYLE17"><a href="https://2008.igem.org/Experiment">Experiment</a></span> </div></td> |
- | <td width="493"><div align="center"><a href="https://2008.igem.org/Modle" class="STYLE18">Model</a></div></td> | + | <td width="493" bgcolor="#03438A"><div align="center"><a href="https://2008.igem.org/Modle" class="STYLE18">Model</a></div></td> |
</tr> | </tr> | ||
</table></td> | </table></td> |
Revision as of 12:26, 29 October 2008
Home | |||||||||||||||||||||||||||||||||||
This idea was inspired by the theory of Prisoner’s Dilemma. As in prisoners’ dilemma, the bacteria in our design are faced with two solutions for coexistence, they could either choose to cooperate with each other by providing inducers to express their partners’ antibiotics-resistance genes or they could take a foe strategy in which no cooperation is needed for both strains’ survival. |
|||||||||||||||||||||||||||||||||||