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Team:iHKU/biobrick
From 2008.igem.org
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<p><u><a name="p20" id="p1"></a></u>BBa_K094100 <img src="https://static.igem.org/mediawiki/2008/6/63/Biobrick_pic1.gif" width="50" height="52" /><br /> | <p><u><a name="p20" id="p1"></a></u>BBa_K094100 <img src="https://static.igem.org/mediawiki/2008/6/63/Biobrick_pic1.gif" width="50" height="52" /><br /> | ||
cheZ: This part contains the cheZ operon coding region without an rbs nor terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.</p> | cheZ: This part contains the cheZ operon coding region without an rbs nor terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p2"></a></u>BBa_K094101 <img src="https://static.igem.org/mediawiki/2008/b/bb/Biobrick_pic2.gif" width="146" height="55" /><br /> | <p><u><a name="p20" id="p2"></a></u>BBa_K094101 <img src="https://static.igem.org/mediawiki/2008/b/bb/Biobrick_pic2.gif" width="146" height="55" /><br /> | ||
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<p><u><a name="p20" id="p3"></a></u>BBa_K094102 <img src="https://static.igem.org/mediawiki/2008/d/d5/Biobrick_pic3.gif" width="122" height="54" /><br /> | <p><u><a name="p20" id="p3"></a></u>BBa_K094102 <img src="https://static.igem.org/mediawiki/2008/d/d5/Biobrick_pic3.gif" width="122" height="54" /><br /> | ||
pTetR-rbs-cheZ: This part uses BBa_J61003 as a backbone and cheZ as the inserted gene. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The cheZ operon lacks a terminator.</p> | pTetR-rbs-cheZ: This part uses BBa_J61003 as a backbone and cheZ as the inserted gene. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The cheZ operon lacks a terminator.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p4"></a></u>BBa_K094103 <img src="https://static.igem.org/mediawiki/2008/c/c2/Biobrick_pic4.gif" width="156" height="56" /><br /> | <p><u><a name="p20" id="p4"></a></u>BBa_K094103 <img src="https://static.igem.org/mediawiki/2008/c/c2/Biobrick_pic4.gif" width="156" height="56" /><br /> | ||
plux-rbs-cheZ-terminator: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.</p> | plux-rbs-cheZ-terminator: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p5"></a></u>BBa_K094104 <img width="227" height="48" src="https://static.igem.org/mediawiki/2008/4/4e/Biobrick_pic5.gif" /><br /> | <p><u><a name="p20" id="p5"></a></u>BBa_K094104 <img width="227" height="48" src="https://static.igem.org/mediawiki/2008/4/4e/Biobrick_pic5.gif" /><br /> | ||
pLacI/ara-1-rbs-mRFP-double terminator: This part is adapted from part BBa_J04450 by changing the promoter pLacI to promoter pLacI/ara-1. This part can test the leaky of promoter plac/ara-1.</p> | pLacI/ara-1-rbs-mRFP-double terminator: This part is adapted from part BBa_J04450 by changing the promoter pLacI to promoter pLacI/ara-1. This part can test the leaky of promoter plac/ara-1.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p6"></a></u>BBa_K094105 <img width="292" height="58" src="https://static.igem.org/mediawiki/2008/9/9d/Biobrick_pic6.gif" /><br /> | <p><u><a name="p20" id="p6"></a></u>BBa_K094105 <img width="292" height="58" src="https://static.igem.org/mediawiki/2008/9/9d/Biobrick_pic6.gif" /><br /> | ||
plambda(R-O12)-rbs-cheZ:-rbs-mRFP1-double terminator: This part can be used to measure the gene expression of cheZ under the control of promoter plambda(R-O12).</p> | plambda(R-O12)-rbs-cheZ:-rbs-mRFP1-double terminator: This part can be used to measure the gene expression of cheZ under the control of promoter plambda(R-O12).</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p7"></a></u>BBa_K094106 <img width="277" height="77" src="https://static.igem.org/mediawiki/2008/9/9b/Biobrick_pic7.gif" /><br /> | <p><u><a name="p20" id="p7"></a></u>BBa_K094106 <img width="277" height="77" src="https://static.igem.org/mediawiki/2008/9/9b/Biobrick_pic7.gif" /><br /> | ||
Plux-rbs-CI-terminator-plambda-rbs-cheZ: This part encodes a CI protein that is used to repress lambda promoter. The part is controlled by the lux promoter that can be induced by luxR protein under high AHL concentration. In this part, the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances, the cheZ gene express constitutively.</p> | Plux-rbs-CI-terminator-plambda-rbs-cheZ: This part encodes a CI protein that is used to repress lambda promoter. The part is controlled by the lux promoter that can be induced by luxR protein under high AHL concentration. In this part, the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances, the cheZ gene express constitutively.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p8"></a></u>BBa_K094110 <img width="51" height="49" src="https://static.igem.org/mediawiki/2008/9/9b/Biobrick_pic8.gif" /><br /> | <p><u><a name="p20" id="p8"></a></u>BBa_K094110 <img width="51" height="49" src="https://static.igem.org/mediawiki/2008/9/9b/Biobrick_pic8.gif" /><br /> | ||
cheY*: This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part contains the coding region only.</p> | cheY*: This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part contains the coding region only.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p9"></a></u>BBa_K094111<img width="122" height="53" src="https://static.igem.org/mediawiki/2008/0/07/Biobrick_pic9.gif" /><br /> | <p><u><a name="p20" id="p9"></a></u>BBa_K094111<img width="122" height="53" src="https://static.igem.org/mediawiki/2008/0/07/Biobrick_pic9.gif" /><br /> | ||
pTetR-rbs-cheY*: This part uses BBa_J61003 as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part lacks a terminator.</p> | pTetR-rbs-cheY*: This part uses BBa_J61003 as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part lacks a terminator.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p10"></a></u>BBa_K094112 <img width="150" height="58" src="https://static.igem.org/mediawiki/2008/3/33/Biobrick_pic10.gif" /><br /> | <p><u><a name="p20" id="p10"></a></u>BBa_K094112 <img width="150" height="58" src="https://static.igem.org/mediawiki/2008/3/33/Biobrick_pic10.gif" /><br /> | ||
luxR-rbs-luxI-terminator: : The bacterial <a href="http://en.wikipedia.org/wiki/Luciferin" title="Luciferin">luciferin</a>-<a href="http://en.wikipedia.org/wiki/Luciferase" title="Luciferase">luciferase</a> system is encoded by a set of genes labelled the <a href="http://en.wikipedia.org/w/index.php?title=Lux_operon&action=edit&redlink=1" title="Lux operon (page does not exist)">Lux operon</a>. In <em>V. fischeri</em> five such genes (<em>LuxCDABE</em>) have been identified as active in the emission of visible light, and two genes (<em>LuxR</em> and <em>LuxI</em>) are involved in regulating the <a href="http://en.wikipedia.org/wiki/Operon" title="Operon">operon</a>. This part can be utilized as a part of quorum sensing system.</p> | luxR-rbs-luxI-terminator: : The bacterial <a href="http://en.wikipedia.org/wiki/Luciferin" title="Luciferin">luciferin</a>-<a href="http://en.wikipedia.org/wiki/Luciferase" title="Luciferase">luciferase</a> system is encoded by a set of genes labelled the <a href="http://en.wikipedia.org/w/index.php?title=Lux_operon&action=edit&redlink=1" title="Lux operon (page does not exist)">Lux operon</a>. In <em>V. fischeri</em> five such genes (<em>LuxCDABE</em>) have been identified as active in the emission of visible light, and two genes (<em>LuxR</em> and <em>LuxI</em>) are involved in regulating the <a href="http://en.wikipedia.org/wiki/Operon" title="Operon">operon</a>. This part can be utilized as a part of quorum sensing system.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p11"></a></u>BBa_K094113<img width="50" height="47" src="https://static.igem.org/mediawiki/2008/1/1b/Biobrick_pic11.gif" /><br /> | <p><u><a name="p20" id="p11"></a></u>BBa_K094113<img width="50" height="47" src="https://static.igem.org/mediawiki/2008/1/1b/Biobrick_pic11.gif" /><br /> | ||
lacZ: This is a reporter gene that codes for beta galactosidase.</p> | lacZ: This is a reporter gene that codes for beta galactosidase.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p12"></a></u>BBa_K094120 <img width="57" height="61" src="https://static.igem.org/mediawiki/2008/b/b4/Biobrick_pic12.gif" /><br /> | <p><u><a name="p20" id="p12"></a></u>BBa_K094120 <img width="57" height="61" src="https://static.igem.org/mediawiki/2008/b/b4/Biobrick_pic12.gif" /><br /> | ||
pLacI/ara-1: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.</p> | pLacI/ara-1: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p13"></a></u>BBa_K094130 <img width="164" height="64" src="https://static.igem.org/mediawiki/2008/c/c6/Biobrick_pic13.gif" /><br /> | <p><u><a name="p20" id="p13"></a></u>BBa_K094130 <img width="164" height="64" src="https://static.igem.org/mediawiki/2008/c/c6/Biobrick_pic13.gif" /><br /> | ||
rbs-mRFP1-terminator: This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.</p> | rbs-mRFP1-terminator: This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p14"></a></u>BBa_K094141 <<img width="171" height="59" src="https://static.igem.org/mediawiki/2008/c/cc/Biobrick_pic14.gif" /><br /> | <p><u><a name="p20" id="p14"></a></u>BBa_K094141 <<img width="171" height="59" src="https://static.igem.org/mediawiki/2008/c/cc/Biobrick_pic14.gif" /><br /> | ||
pLacIq-rbs-LacIq-terminator: LacIQ can repress promoter lac. This part contains a mutated pLacIq promoter which enables high level of transcription. This ensures effective repression of pLacI promoter that might be present. </p> | pLacIq-rbs-LacIq-terminator: LacIQ can repress promoter lac. This part contains a mutated pLacIq promoter which enables high level of transcription. This ensures effective repression of pLacI promoter that might be present. </p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p15"></a></u>BBa_K094150 <img width="227" height="62" src="https://static.igem.org/mediawiki/2008/0/0f/Biobrick_pic15.gif" /><br /> | <p><u><a name="p20" id="p15"></a></u>BBa_K094150 <img width="227" height="62" src="https://static.igem.org/mediawiki/2008/0/0f/Biobrick_pic15.gif" /><br /> | ||
pLacI/ara-1-rbs-luxI-luxR-terminator: This is a quorum sensing composition. Induced by IPTG or arabinose, this part can establish a quorum sensing system, with the luxI protein producing AHL and luxR protein as the AHL density sensor.</p> | pLacI/ara-1-rbs-luxI-luxR-terminator: This is a quorum sensing composition. Induced by IPTG or arabinose, this part can establish a quorum sensing system, with the luxI protein producing AHL and luxR protein as the AHL density sensor.</p> | ||
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<p> </p> | <p> </p> | ||
<p><u><a name="p20" id="p16"></a></u>BBa_K094151 <img width="162" height="61" src="https://static.igem.org/mediawiki/2008/4/4d/Biobrick_pic16.gif" /><br /> | <p><u><a name="p20" id="p16"></a></u>BBa_K094151 <img width="162" height="61" src="https://static.igem.org/mediawiki/2008/4/4d/Biobrick_pic16.gif" /><br /> | ||
Revision as of 13:36, 29 October 2008
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BiobrickList of Biobricks:BBa_K094100, BBa_K094101, BBa_K094102, BBa_K094103, BBa_K094104, BBa_K094105, BBa_K094106, BBa_K094110, BBa_K094111, BBa_K094112, BBa_K094113, BBa_K094120, BBa_K094130, BBa_K094141, BBa_K094150, BBa_K094151
BBa_K094100
BBa_K094101
BBa_K094102
BBa_K094103
BBa_K094104
BBa_K094105
BBa_K094106
BBa_K094110
BBa_K094111
BBa_K094112
BBa_K094113
BBa_K094120
BBa_K094130
BBa_K094141 <
BBa_K094150
BBa_K094151
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