Team:Edinburgh/Plan/Beta-Carotene

From 2008.igem.org

(Difference between revisions)
(β-carotene synthesis)
(Directly involved genes from P. ananatis:)
Line 5: Line 5:
β-carotene is produced from the products of glycolysis, as can be seen in the overview figure. We have concentrated on two areas of β-carotene for our project. The first involves transfering genes from ''Pantoea ananatis'', a member of the proteobacteria naturally capable of synthesising β-carotene. The second involves upregulating the glycolysis pathways in ''E. coli'' in order to concentrate more energy into making β-carotene.
β-carotene is produced from the products of glycolysis, as can be seen in the overview figure. We have concentrated on two areas of β-carotene for our project. The first involves transfering genes from ''Pantoea ananatis'', a member of the proteobacteria naturally capable of synthesising β-carotene. The second involves upregulating the glycolysis pathways in ''E. coli'' in order to concentrate more energy into making β-carotene.
-
== Directly involved genes from ''P. ananatis'': ==
+
== Directly involved genes from ''P. ananatis'' ==
    
    
# Geranyl diphosphate synthase ([https://2008.igem.org/Team:Edinburgh/crtE '''''crtE''''']) converts the substrates farnesyl diphosphate and isopentyl diphosphate into geranyl geranyl diphosphate.
# Geranyl diphosphate synthase ([https://2008.igem.org/Team:Edinburgh/crtE '''''crtE''''']) converts the substrates farnesyl diphosphate and isopentyl diphosphate into geranyl geranyl diphosphate.

Revision as of 15:22, 29 October 2008

Contents

β-carotene synthesis

β-carotene is produced from the products of glycolysis, as can be seen in the overview figure. We have concentrated on two areas of β-carotene for our project. The first involves transfering genes from Pantoea ananatis, a member of the proteobacteria naturally capable of synthesising β-carotene. The second involves upregulating the glycolysis pathways in E. coli in order to concentrate more energy into making β-carotene.

Directly involved genes from P. ananatis

  1. Geranyl diphosphate synthase (crtE) converts the substrates farnesyl diphosphate and isopentyl diphosphate into geranyl geranyl diphosphate.
  2. Geranyl geranyl diphosphate is then converted into phytoene by phytoene synthase (crtB).
  3. Lycopene is produced from phytoene by phytoene desaturase (crtI).
  4. Finally, lycopene β-cyclase (crtY) cyclises lycopene to produce β-carotene.

Indirectly involved genes from E. coli

Overview

Edinburgh-Beta-carotene.jpg
In blue are the genes which we manipulated.