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Team:Edinburgh/Plan/Beta-Carotene
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== Directly involved genes from ''P. ananatis'' == | == Directly involved genes from ''P. ananatis'' == | ||
| - | [[Image:Edinburgh-CarotenoidStructures.jpg|thumb|200px|right|'''Figure 1:''' Details of the β-carotene synthesis pathway, including structures.]] | + | [[Image:Edinburgh-CarotenoidStructures.jpg|thumb|200px|right|'''Figure 1:''' Details of the β-carotene synthesis pathway, including structures and genes involved.]] |
# Geranyl diphosphate synthase ([https://2008.igem.org/Team:Edinburgh/crtE '''''crtE''''']) converts the substrates farnesyl diphosphate and isopentyl diphosphate into geranyl geranyl diphosphate. | # Geranyl diphosphate synthase ([https://2008.igem.org/Team:Edinburgh/crtE '''''crtE''''']) converts the substrates farnesyl diphosphate and isopentyl diphosphate into geranyl geranyl diphosphate. | ||
Revision as of 16:22, 29 October 2008
Contents |
β-carotene synthesis
β-carotene is produced from the products of glycolysis, as can be seen in the overview figure. We have concentrated on two areas of β-carotene for our project. The first involves transfering carotenoid synthesis genes from Pantoea ananatis, a member of the proteobacteria naturally capable of producing β-carotene. The second involves upregulating the glycolysis pathways in E. coli in order to concentrate more energy into making β-carotene.
Directly involved genes from P. ananatis
- Geranyl diphosphate synthase (crtE) converts the substrates farnesyl diphosphate and isopentyl diphosphate into geranyl geranyl diphosphate.
- Geranyl geranyl diphosphate is then converted into phytoene by phytoene synthase (crtB).
- Lycopene is produced from phytoene by phytoene desaturase (crtI).
- Finally, lycopene β-cyclase (crtY) cyclises lycopene to produce β-carotene.
See figure 1 for structure details.
Indirectly involved genes from E. coli
Overview
- In blue are the genes which we manipulated.
