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Team:Heidelberg/Notebook/Visualization/Notebook/SwarmAssays
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Petri-dishes were used es running chambers.<br> | Petri-dishes were used es running chambers.<br> | ||
A petri-dish was filled with a media containig agar. Then the attractant was spotted on premarked positions or spotted as a line through the center of the petri-dish. After the gradient of the attractant has etablished the strains whioch should be attracted by the attractant where also set on premarked positions. The whole petri-dish was incubated at 37 °C and pictures were taken after 24, 48 and 72 hours.<br> | A petri-dish was filled with a media containig agar. Then the attractant was spotted on premarked positions or spotted as a line through the center of the petri-dish. After the gradient of the attractant has etablished the strains whioch should be attracted by the attractant where also set on premarked positions. The whole petri-dish was incubated at 37 °C and pictures were taken after 24, 48 and 72 hours.<br> | ||
| - | For a more detailed protocol visit the [https://2008.igem.org/Team:Heidelberg/Notebook/Visualization/Methods | + | For a more detailed protocol visit the [https://2008.igem.org/Team:Heidelberg/Notebook/Visualization/Methods Methods] page.<br> |
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| + | ===First swarm plates=== | ||
| + | |||
| + | A swarm assay test in 4 swarm plates was prepared.<br> | ||
| + | 2 different fusion constructs were used. Each in HCB33 and in UU1250.<br> | ||
| + | Every petri-dish was filled with 20 ml of media. Two with minimal media containing about 0.3 % agar and the other two with TB media containing 0.2 % agar.<br> | ||
| + | |||
| + | On a center line of both minimal plates and on one of the TB plates two spots of DH5α were created.<br> | ||
| + | Afterwards fusion contruct 1 containing bacteria HCB33 and UU1250 were each spotted on the right half with an equal distance to the attractant spots. The same was done with the fusion construct 2 containing bacteria strains, but on the other half of the plate.<br> | ||
[https://2008.igem.org/Team:Heidelberg/Notebook/visualization back to the visualization notebook] | [https://2008.igem.org/Team:Heidelberg/Notebook/visualization back to the visualization notebook] | ||
Revision as of 16:33, 29 October 2008
Swarm Assays
STARTED 2008-09-27
PURPOSE:
Test of the abbility of strains expressing the fusion receptor, to move against an attractant gradient.
STRAIN:
As autoinducer 2 producing attactant strain DH5α was used.
The fusion receptor plasmid was further cloned into HCB33 and UU1250 strains.
METHOD:
Petri-dishes were used es running chambers.
A petri-dish was filled with a media containig agar. Then the attractant was spotted on premarked positions or spotted as a line through the center of the petri-dish. After the gradient of the attractant has etablished the strains whioch should be attracted by the attractant where also set on premarked positions. The whole petri-dish was incubated at 37 °C and pictures were taken after 24, 48 and 72 hours.
For a more detailed protocol visit the Methods page.
A few pictures which have been taken during these experiments can be viewed under Projects.
First swarm plates
A swarm assay test in 4 swarm plates was prepared.
2 different fusion constructs were used. Each in HCB33 and in UU1250.
Every petri-dish was filled with 20 ml of media. Two with minimal media containing about 0.3 % agar and the other two with TB media containing 0.2 % agar.
On a center line of both minimal plates and on one of the TB plates two spots of DH5α were created.
Afterwards fusion contruct 1 containing bacteria HCB33 and UU1250 were each spotted on the right half with an equal distance to the attractant spots. The same was done with the fusion construct 2 containing bacteria strains, but on the other half of the plate.
back to the visualization notebook
