Team:Brown/Notebook/Timeline
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*Different concentrations of bacterial cells tested with varying amounts of Arabinose. The control of these tests was either a pVJ4 culture without added Arabinose or a plasmid containing the Cell Lysis Cassette under a different promoter, for example pRG1 - on a P''lac'' plasmid. | *Different concentrations of bacterial cells tested with varying amounts of Arabinose. The control of these tests was either a pVJ4 culture without added Arabinose or a plasmid containing the Cell Lysis Cassette under a different promoter, for example pRG1 - on a P''lac'' plasmid. | ||
- | ====Week Nine==== | + | ====Week Nine +==== |
- | *Wrap up of the summer. | + | *Wrap up of the summer. Work resumed in September. |
- | *More testing with NaCl and Milli-Q water. | + | *More sensitivity testing with NaCl and Milli-Q water. |
- | *Made the switch to conductivity measurements. Obtained a | + | *Made the switch to conductivity measurements. Obtained a conductivity probe and obtained beautiful results. Cells were not concentrated. Arabinose added and probe left in solution for four hours. A steep curve resulted! |
+ | *Much of our efforts were concentrated on Biobricking our different parts. Team Toxipop had problems over the summer obtaining PCR products. We were finally able to Biobrick the pVJ4 plasmid, the S105 gene, and the lysis cassette with S105,R,Rz genes. The pVJ4 plasmid contains the Cell Lysis Cassette under the control of an Arabinose Inducible Promoter. All tests run throughout the summer were done with pVJ4. The team received the S105 cassette and gene in September from Ry Young's Lab at Texas A&M. |
Latest revision as of 17:35, 29 October 2008
Timeline as presented to Faculty Mentors and Graduate Advisors in May:
Actual TimelineWeek One
Week Two
Week Three
Week Four
Week Five
Week Six
Week Seven
Week Eight
Week Nine +
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