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Team:Chiba/Calendar-Home/28 August 2008
From 2008.igem.org
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| - | '''[[Team:Chiba/protocol/digestion|Digestion]]''' | + | '''[[Team:Chiba/protocol/digestion|Digestion]]''' 37°C、30分 |
<BR>プラスミドの濃度は33.6ng/μl | <BR>プラスミドの濃度は33.6ng/μl | ||
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| - | -->濃度は33.6ng/ | + | -->濃度は33.6ng/μl |
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===Team:Communication=== | ===Team:Communication=== | ||
Revision as of 18:27, 29 October 2008
27 August 2008 <|> 29 August 2008
Contents |
Laboratory work
Team:Input
[http://partsregistry.org/Part:BBa_I7106 BBa_I7106](2007) 生えてきたコロニーを10ml培養してMini prep(60mlで溶出):
-->Digestion
混ぜ表
| double | |
| dH2O | 2 |
| 10×BSA | 1 |
| 10×NE | 1 |
| EcoRI | 0.5 |
| PstI | 0.5 |
| DNA | 5 |
| TOTAL | 10 |
Digestion 37°C、30分
プラスミドの濃度は33.6ng/μl
PCR
RBS＋CI
混ぜ表
| DNA | 1 |
| FW primer | 2.5 |
| VR primer | 2.5 |
| dNTPmix | 5 |
| thermo pol buffer | 5 |
| DNA pol(VENT) | 1 |
| dH2O | 34 |
| TOTAL | 50 |
| PCR産物 | 1 |
| Dye | 1 |
| dH2O | 4 |
| TOTAL | 6 |
-->濃度は33.6ng/μl
ベクターのPCR
[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
混ぜ表
| DNA | 1 |
| FW primer | 5 |
| VR primer | 5 |
| dNTPmix | 10 |
| thermo pol buffer | 10 |
| DNA pol(VENT) | 1 |
| dH2O | 68 |
| TOTAL | 100 |
| PCR産物 | 1 |
| Dye | 1 |
| dH2O | 4 |
| TOTAL | 6 |
-->バンド出ず。
Team:Communication
- (27/8)--->Mini prep
- insert:C0170 + vector:J04500
- insert:C0178 + vector:J04500
- --->Digestion Test
- insert:C0170 + vector:J04500
- insert:C0178 + vector:J04500
| Sample No. | 3 | 4 | 5 | 6 |
| insert:C0170 + vector:J04500 | 1 | - | 1 | - |
| insert:C0178 + vector:J04500 | - | 1 | - | 1 |
| EcoRⅠ | 0.1 | 0.1 | 0.1 | 0.1 |
| SpeⅠ | - | - | 0.1 | 0.1 |
| Buffer 3 | 1 | 1 | 1 | 1 |
| BSA | - | - | 1 | 1 |
| dH2O | 7.8 | 7.8 | 6.8 | 6.8 |
| TOTAL | 10 | 10 | 10 | 10 |
- --->(29/8)Gel Check
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