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Team:Chiba/Calendar-Home/28 August 2008
From 2008.igem.org
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<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>10μl </td> |
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<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>50μl </td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>6μl </td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>100μl </td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>6μl </td> |
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</table> | </table> | ||
Revision as of 18:51, 29 October 2008
27 August 2008 <|> 29 August 2008
Contents |
Laboratory work
Team:Input
[http://partsregistry.org/Part:BBa_I7106 BBa_I7106](2007) 生えてきたコロニーを10ml培養してMini prep(60mlで溶出)
-->Digestion
混ぜ表
| double | |
| dH2O | 2 |
| 10×BSA | 1 |
| 10×NE | 1 |
| EcoRI | 0.5 |
| PstI | 0.5 |
| DNA | 5 |
| TOTAL | 10μl |
Digestion 37°C、30分
プラスミドの濃度は33.6ng/μl
PCR
RBS＋CI
混ぜ表
| DNA | 1 |
| FW primer | 2.5 |
| VR primer | 2.5 |
| dNTPmix | 5 |
| thermo pol buffer | 5 |
| DNA pol(VENT) | 1 |
| dH2O | 34 |
| TOTAL | 50μl |
| PCR産物 | 1 |
| Dye | 1 |
| dH2O | 4 |
| TOTAL | 6μl |
-->濃度は33.6ng/μl
ベクターのPCR
[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
混ぜ表
| DNA | 1 |
| FW primer | 5 |
| VR primer | 5 |
| dNTPmix | 10 |
| thermo pol buffer | 10 |
| DNA pol(VENT) | 1 |
| dH2O | 68 |
| TOTAL | 100μl |
| PCR産物 | 1 |
| Dye | 1 |
| dH2O | 4 |
| TOTAL | 6μl |
-->バンド出ず。
Team:Communication
- (27/8)--->Mini prep
- insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
- insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
- --->Digestion Test
- insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
- insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
| Sample No. | 3 | 4 | 5 | 6 |
| insert:C0170 + vector:J04500 | 1 | - | 1 | - |
| insert:C0178 + vector:J04500 | - | 1 | - | 1 |
| EcoRⅠ | 0.1 | 0.1 | 0.1 | 0.1 |
| SpeⅠ | - | - | 0.1 | 0.1 |
| Buffer 3 | 1 | 1 | 1 | 1 |
| BSA | - | - | 1 | 1 |
| dH2O | 7.8 | 7.8 | 6.8 | 6.8 |
| TOTAL | 10 | 10 | 10 | 10 |
- --->(29/8)Gel Check
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