Team:Paris/Modeling/Workflow Example
From 2008.igem.org
(Difference between revisions)
Line 4: | Line 4: | ||
Here, we will explain the generic protocol that leads to the modelisation "by characterization", of one of the parts of our system. The choosen example is '''the ACTIVITY of the PROMOTER pFliL''' (that leads to the production of the '''Fluorescent Protein FP1''') '''in function of the TRANSCRIPTION FACTORS FlhDC and FliA'''. | Here, we will explain the generic protocol that leads to the modelisation "by characterization", of one of the parts of our system. The choosen example is '''the ACTIVITY of the PROMOTER pFliL''' (that leads to the production of the '''Fluorescent Protein FP1''') '''in function of the TRANSCRIPTION FACTORS FlhDC and FliA'''. | ||
We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br> | We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br> | ||
- | As it is illustrated below; starting from | + | As it is illustrated below; starting from ''molecular reactions'' '''(1)''', we develop a ''model of our system'' '''(2)''', and then we identify the parameters '''(3)''' that needs to be estimated. Essentially, it amounts to estimate <span style="color:#0000FF;">Promoters Activities as functions of their Transcription Factors</span>. To do so, we propose ''constructs</span>'' '''(4)''', ''protocols'' '''(5)''' and ''programs'' '''(6)'''. |
<br> | <br> | ||
<center><small>''You can click on the images or in the button below to get further informations on the represented step''</small></center> | <center><small>''You can click on the images or in the button below to get further informations on the represented step''</small></center> |
Revision as of 19:20, 29 October 2008