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Alberta NINT/25 June 2008
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(New page: lab work (JD): Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be cut better when looking at gels and ligating them to the new...) |
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| + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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lab work (JD): | lab work (JD): | ||
Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I still haven't tried) | Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I still haven't tried) | ||
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Transformed XL1-B cells with the ligations and negative controls | Transformed XL1-B cells with the ligations and negative controls | ||
Plated cells | Plated cells | ||
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| + | [[Alberta_NINT/26_June_2008 | Next entry>]] | ||
Revision as of 21:09, 26 June 2008
lab work (JD):
Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I still haven't tried)
Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed
Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed
Created K102006: K102003/B+N and TA0/B+N newly annealed
Transformed XL1-B cells with the ligations and negative controls
Plated cells
