Team:Chiba/Calendar-Home/12 September 2008

From 2008.igem.org

(Difference between revisions)
(Team:Input)
(Team:Input)
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*Pbad/araC:L-Arabinose induction test
*Pbad/araC:L-Arabinose induction test
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*BBa_I1304(Ptet-RBS-GFP,XL10G)×1()(negative control)
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*[http://partsregistry.org/Part:BBa_I1304 BBa_I1304]×1(1)(negative control)
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*BBa_R0051(PcI)×4(②~⑤)Select single colony from Agar Plate and introduce to 2mlLB each other.  
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*[http://partsregistry.org/Part:BBa_R0051 BBa_R0051]×4(1)~(5)Select single colony from Agar Plate and introduce to 2mlLB each other.  
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Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6).
Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6).
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L-アラビノースを入れてから15min(?30min?),1h,2h,6h,12h,24h後に蛍光強度を計測した。
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L-アラビノースを入れてから15min(30min),1h,2h,6h,12h,24h後に蛍光強度を計測した。

Revision as of 19:40, 29 October 2008

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11 September 2008 <|> 13 September 2008

Contents

Laboratory work

Team:Input

  • Pbad/araC:L-Arabinose induction test
  • [http://partsregistry.org/Part:BBa_I1304 BBa_I1304]×1(1)(negative control)
  • [http://partsregistry.org/Part:BBa_R0051 BBa_R0051]×4(1)~(5)Select single colony from Agar Plate and introduce to 2mlLB each other.


  1. incubated cultures at 37 &degC; for 12hours.
  2. Measured OD600.


Reaction mixtures were mixed in 48-well plate(deep well).

混ぜ表

Sample No. 12345
Cultures(μL) 0μl1.9μl19μl190μl-
20%L-Arabinose(μL) 0μl1.9μl19μl190μl-
L-Arabinase conc. 0%(N.C)0.2%2%20%P.C

Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6).

L-アラビノースを入れてから15min(30min),1h,2h,6h,12h,24h後に蛍光強度を計測した。


Result:

Time after arabinose induction / Sample No. 12345
30min 8.8909.75210.109.4139.701
1h 9.16113.9317.3714.9710.60
2h 9.41727.9640.2529.8710.72
6h 9.93374.99150.9125.010.04
12h 10.6499.39202.8872.0811.76
24h 14.66105.6447.333.3117.11

Team:Communication

no work

Team:Output

no work