Edinburgh/27 July 2008

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Latest revision as of 20:37, 29 October 2008

Edinburgh iGEM 2008

June
M	T	W	T	F	S	S
						[http://2008.igem.org/wiki/index.php?title=Edinburgh/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_June_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/3_June_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/4_June_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/5_June_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/6_June_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/7_June_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/9_June_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/10_June_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/11_June_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/12_June_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/13_June_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/14_June_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/16_June_2008&action=edit 16]	[http://2008.igem.org/Edinburgh/17_June_2008 17]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/18_June_2008&action=edit 18]	[http://2008.igem.org/Edinburgh/19_June_2008 19]	[http://2008.igem.org/Edinburgh/20_June_2008 20]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/21_June_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_June_2008&action=edit 23]	[http://2008.igem.org/Edinburgh/24_June_2008 24]	[http://2008.igem.org/Edinburgh/25_June_2008 25]	[http://2008.igem.org/Edinburgh/26_June_2008 26]	[http://2008.igem.org/Edinburgh/27_June_2008 27]	[http://2008.igem.org/Edinburgh/28_June_2008 28]	[http://2008.igem.org/Edinburgh/29_June_2008 29]
[http://2008.igem.org/Edinburgh/30_June_2008 30]

July
M	T	W	T	F	S	S
	[http://2008.igem.org/Edinburgh/1_July_2008 1]	[http://2008.igem.org/Edinburgh/2_July_2008 2]	[http://2008.igem.org/Edinburgh/3_July_2008 3]	[http://2008.igem.org/Edinburgh/4_July_2008 4]	[http://2008.igem.org/Edinburgh/5_July_2008 5]	[http://2008.igem.org/Edinburgh/6_July_2008 6]
[http://2008.igem.org/Edinburgh/7_July_2008 7]	[http://2008.igem.org/Edinburgh/8_July_2008 8]	[http://2008.igem.org/Edinburgh/9_July_2008 9]	[http://2008.igem.org/Edinburgh/10_July_2008 10]	[http://2008.igem.org/Edinburgh/11_July_2008 11]	[http://2008.igem.org/Edinburgh/12_July_2008 12]	[http://2008.igem.org/Edinburgh/13_July_2008 13]
[http://2008.igem.org/Edinburgh/14_July_2008 14]	[http://2008.igem.org/Edinburgh/15_July_2008 15]	[http://2008.igem.org/Edinburgh/16_July_2008 16]	[http://2008.igem.org/Edinburgh/17_July_2008 17]	[http://2008.igem.org/Edinburgh/18_July_2008 18]	[http://2008.igem.org/Edinburgh/19_July_2008 19]	[http://2008.igem.org/Edinburgh/20_July_2008 20]
[http://2008.igem.org/Edinburgh/21_July_2008 21]	[http://2008.igem.org/Edinburgh/22_July_2008 22]	[http://2008.igem.org/Edinburgh/23_July_2008 23]	[http://2008.igem.org/Edinburgh/24_July_2008 24]	[http://2008.igem.org/Edinburgh/25_July_2008 25]	[http://2008.igem.org/Edinburgh/26_July_2008 26]	[http://2008.igem.org/Edinburgh/27_July_2008 27]
[http://2008.igem.org/Edinburgh/28_July_2008 28]	[http://2008.igem.org/Edinburgh/29_July_2008 29]	[http://2008.igem.org/Edinburgh/30_July_2008 30]	[http://2008.igem.org/Edinburgh/31_July_2008 31]

August
M	T	W	T	F	S	S
				[http://2008.igem.org/Edinburgh/1_August_2008 1]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_August_2008&action=edit 2]	[http://2008.igem.org/Edinburgh/3_August_2008 3]
[http://2008.igem.org/Edinburgh/4_August_2008 4]	[http://2008.igem.org/Edinburgh/5_August_2008 5]	[http://2008.igem.org/Edinburgh/6_August_2008 6]	[http://2008.igem.org/Edinburgh/7_August_2008 7]	[http://2008.igem.org/Edinburgh/8_August_2008 8]	[http://2008.igem.org/Edinburgh/9_August_2008 9]	[http://2008.igem.org/Edinburgh/10_August_2008 10]
[http://2008.igem.org/Edinburgh/11_August_2008 11]	[http://2008.igem.org/Edinburgh/12_August_2008 12]	[http://2008.igem.org/Edinburgh/13_August_2008 13]	[http://2008.igem.org/Edinburgh/14_August_2008 14]	[http://2008.igem.org/Edinburgh/15_August_2008 15]	[http://2008.igem.org/Edinburgh/16_August_2008 16]	[http://2008.igem.org/Edinburgh/17_August_2008 17]
[http://2008.igem.org/Edinburgh/18_August_2008 18]	[http://2008.igem.org/Edinburgh/19_August_2008 19]	[http://2008.igem.org/Edinburgh/20_August_2008 20]	[http://2008.igem.org/Edinburgh/21_August_2008 21]	[http://2008.igem.org/Edinburgh/22_August_2008 22]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_August_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/24_August_2008&action=edit 24]
[http://2008.igem.org/Edinburgh/25_August_2008 25]	[http://2008.igem.org/Edinburgh/26_August_2008 26]	[http://2008.igem.org/Edinburgh/27_August_2008 27]	[http://2008.igem.org/Edinburgh/28_August_2008 28]	[http://2008.igem.org/Edinburgh/29_August_2008 29]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/30_August_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/31_August_2008&action=edit 31]

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Week 6

Sunday 27 July 08

Result of transformations: no growth for L19 (pSB1A2+rbs+dxs), L20 (pSB1A2+rbs+appY) or L21 (pSB1A2+rbs+crtB). For L22 (pSB1A2+rbs+crtI) there are some blue and white colonies. For L23 (pSB1A2+P_zntA) there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For L24 (pSB1A2+crtE) there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)

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@@ Line 6: / Line 6: @@
 === Sunday 27 July 08 ===
-* Result of transformations: no growth for L19 (pSB1A2+rbs+''dxs''), L20 (pSB1A2+rbs+''appY'') or L21 (pSB1A2+rbs+''crtB''). For L22 (pSB1A2+rbs+''crtI'') there are some blue and white colonies. For L23 (pSB1A2+pZntA) there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For L24 (pSB1A2+''crtE'') there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)<br />
+* Result of transformations: no growth for L19 (pSB1A2+rbs+''dxs''), L20 (pSB1A2+rbs+''appY'') or L21 (pSB1A2+rbs+''crtB''). For L22 (pSB1A2+rbs+''crtI'') there are some blue and white colonies. For L23 (pSB1A2+''P<sub>zntA</sub>'') there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For L24 (pSB1A2+''crtE'') there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)<br />
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