Team:Chiba/Calendar-Home/13 September 2008

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Revision as of 21:38, 29 October 2008

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12 September 2008 <|> 14 September 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

Time Delay Test

time-delay test
- transformants

receiver

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908)

sender

Plac-RhlI (JW1908)-Amp
Plac-LasI (JW1908)-Amp
Ptet-LuxI (JW1908)-Amp

48-well plate(Deep Well)

1:2ml	2:2ml,AHL	2:2ml	3:2ml	4:2ml	-
-	-	-	-	-	-
1:1ml,2:1ml,IPTG:2μl	1:100μl,2:1ml,IPTG:1.1μl	110μl,21ml,IPTG:101μl	1:1μl,21ml,IPTG:1.001μl	1:1ml,2:100μl,IPTG:1.1μl	1:1ml,2:10μl,IPTG:1.01μl
-	-	-	-	-	-
1:1ml,3:1ml,IPTG:2μl	1:100μl,3:1ml,IPTG:1.1μl	1:10μl,3:1ml,IPTG:1.01μl	1:1μl,3:1ml,IPTG	1:1ml,3:100μl,IPTG:1.1μl	1:1ml,3:10μl,IPTG:1.01μl
-	-	-	-	-	-
1:1ml,4:1ml,IPTG:2μl	1:100μl,4:1ml,IPTG:1.1μl	1:10μl,4:1ml,IPTG:1.0μl	1:1μl,4:1ml,IPTG:1.001μl	1:1ml,4:100μl,IPTG:1.0μl	1:1ml,4:10μl,IPTG1.01μl
-	-	-	-	-	-

Team:Communication

Gel Check

[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

Sample No.	1~3
Sample DNA(μL)	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

From left;

-->Digestion

[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

Sample No.	①	②	③	④
Sample DNA(μL)	5	5	1	1
EcoRⅠ(μL)	0.5	o.5	-	-
SpeⅠ(μL)	0.5	0.5	-	-
Buffer 1(μL)	1	1	-	-
Buffer 3(μL)	-	-	1	-
BSA(μL)	1	1	-	-
dH₂O(μL)	2	2	7.5	-
TOTAL(μL)	10	10	10	1

Team:Output

no work

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