Team:Hawaii/Parts
From 2008.igem.org
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| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs) | | pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs) | ||
| plasmid | | plasmid | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010'''] | ||
+ | | Broad-Host-Range plasmid derived from RSF1010 | ||
+ | | plasmid | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020'''] | ||
+ | | rep/oriv/aada/orit | ||
+ | | intermediate for RSF1010 derived plasmid | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100'''] | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100'''] | ||
Line 59: | Line 67: | ||
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence | | cyanobacterial slr2016 derived amino terminus protein secretion signal sequence | ||
| protein coding | | protein coding | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320'''] | ||
+ | | oriT derived from RP4 | ||
+ | | origin of transfer | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350'''] | ||
+ | | oriTr/LacZ | ||
+ | | conjugation screening | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125360 '''K125360'''] | ||
+ | | oriT/LacZ | ||
+ | | conjugation screening | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500'''] | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500'''] | ||
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| protein coding | | protein coding | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
- | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K125520'''] | |
- | + | ||
- | + | ||
- | + | ||
- | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 ''' | + | |
| aadA gene | | aadA gene | ||
| protein coding | | protein coding | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
- | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 ''' | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K125530'''] |
| aadA gene with promoter and rbs | | aadA gene with promoter and rbs | ||
| protein coding | | protein coding | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800'''] | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800'''] | ||
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| protein coding | | protein coding | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
- | | [http://partsregistry.org/wiki/index.php/Part: | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810'''] |
- | | | + | | N-terminal slr2016 signal sequence attached to GFP fusion protein |
- | | | + | | protein coding |
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
- | | [http://partsregistry.org/wiki/index.php/Part: | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820'''] |
- | | | + | | ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter |
- | + | | device | |
- | + | ||
- | + | ||
- | + | ||
- | | | + | |
|} | |} | ||
==Parts submitted== | ==Parts submitted== |
Revision as of 22:04, 29 October 2008
Contents |
Guidelines for naming parts
Part number | Part type |
---|---|
K125000-K125099 | plasmid |
K125100-K125199 | promoter |
K125200-K125299 | ribosome binding site |
K125300-K125499 | signal sequences, conjugation sequences, random stuff |
K125500-K125699 | protein coding |
K125700-K125799 | transcription terminator |
K125800-K125999 | devices |
Parts planned
Parts in process (building)
Part number | Description | Type |
---|---|---|
[http://partsregistry.org/wiki/index.php/Part:BBa_K125000 K125000] | pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs) | plasmid |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125010 K125010] | Broad-Host-Range plasmid derived from RSF1010 | plasmid |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125020 K125020] | rep/oriv/aada/orit | intermediate for RSF1010 derived plasmid |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100] | nitrate inducible nir promoter | regulator |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125110] | nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] | regulator |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125120] | lac promoter + ribosome binding site with efficiency of 1.0 (composite of I14032 and B0030) | regulator |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125300 K125300] | cyanobacterial pilA derived amino termninus protein secretion signal sequence | protein coding |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310] | cyanobacterial slr2016 derived amino terminus protein secretion signal sequence | protein coding |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125320 K125320] | oriT derived from RP4 | origin of transfer |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125350 K125350] | oriTr/LacZ | conjugation screening |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125360 K125360] | oriT/LacZ | conjugation screening |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500] | GFP fusion protein | protein coding |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125520 K125520] | aadA gene | protein coding |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125530 K125530] | aadA gene with promoter and rbs | protein coding |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125800 K125800] | rep proteins with promoter and rbs | protein coding |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125810 K125810] | N-terminal slr2016 signal sequence attached to GFP fusion protein | protein coding |
[http://partsregistry.org/wiki/index.php/Part:BBa_K125820 K125820] | Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter | device |