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Team:Chiba/Calendar-Home/19 September 2008
From 2008.igem.org
(Difference between revisions)
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<tr> | <tr> | ||
<td width="257">Sample Name</td> | <td width="257">Sample Name</td> | ||
| - | <td>J04500</td><td>R0010</td><td>C0161</td><td>Cin</td> | + | <td>[http://partsregistry.org/Part:BBa_J04500 J04500]</td><td>[http://partsregistry.org/Part:BBa_R0010 R0010]</td><td>[http://partsregistry.org/Part:BBa_C0161 C0161]</td><td>Cin</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 31: | Line 31: | ||
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]''' | :--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]''' | ||
*elution | *elution | ||
| - | ** | + | **[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]-->27μL |
| - | ** | + | **[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]-->11μL |
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | --->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | ||
| Line 43: | Line 43: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>C0161(μL)</td> | + | <td>[http://partsregistry.org/Part:BBa_C0161 C0161](μL)</td> |
<td>3</td><td>3</td><td>-</td> | <td>3</td><td>3</td><td>-</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>J04500(μL)</td> | + | <td>[http://partsregistry.org/Part:BBa_J04500 J04500](μL)</td> |
<td>4.5</td><td>-</td><td>4.5</td> | <td>4.5</td><td>-</td><td>4.5</td> | ||
</tr> | </tr> | ||
Revision as of 22:42, 29 October 2008
18 September 2008 <|> 20 September 2008
Laboratory work
Team:Communication
-->(?/9) Gel Check
| Sample Name | [http://partsregistry.org/Part:BBa_J04500 J04500] | [http://partsregistry.org/Part:BBa_R0010 R0010] | [http://partsregistry.org/Part:BBa_C0161 C0161] | Cin |
| Sample DNA(μL) | 30 | 30 | 90 | ? |
| Loading Dye(μL) | 6 | 6 | 18 | ? |
| TOTAL(μL) | 36 | 36 | 108 | 24 |
--->Gel Extract
(3 wells for 1 sample)
- note:ADB buffer添加後,left at 37°C,for 6 hours
- --->Zymo Clean
- elution
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500]-->27μL
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161]-->11μL
--->Gel Check
- --->Ligation
| Sample Name | insert+vector | insert | vector |
| [http://partsregistry.org/Part:BBa_C0161 C0161](μL) | 3 | 3 | - |
| [http://partsregistry.org/Part:BBa_J04500 J04500](μL) | 4.5 | - | 4.5 |
| ligase(μL) | 1 | 1 | 1 |
| Buffer(invitrogen)(μL) | 3 | 2 | 2 |
| NFW(μL) | 3.5 | 4 | 2.5 |
| TOTAL(μL) | 15 | 10 | 10 |
- --->Transformed above ligation onto a LB-amp plate.
- note
- competent cell:XL10GOLD
- DNA:1μL,SOC:120μL
--->(20/9)Mini prep
- time-delay test
- conditions
- strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
30°C and Room Temparature
Team:Output
colony PCR
ptet+LuxR(×16)
ptet+LasR(×7)
ptet+RhlR(×5)
ptet+cinR(×5)
