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Minnesota/26 June 2008
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(New page: {| |- |1. '''Glycerol Stock:''' |- |a. Add 900uL of glycerol to empty tube |- |b. Add 900uL of cells to the glycerol tubes |- |c. Immediately place tubes in a beaker that has ethanol and ...)
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(New page: {| |- |1. '''Glycerol Stock:''' |- |a. Add 900uL of glycerol to empty tube |- |b. Add 900uL of cells to the glycerol tubes |- |c. Immediately place tubes in a beaker that has ethanol and ...)
Newer edit →
Revision as of 18:48, 27 June 2008
| 1. Glycerol Stock: |
| a. Add 900uL of glycerol to empty tube |
| b. Add 900uL of cells to the glycerol tubes |
| c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. |
| d. Place tubes in freezer @ -79C |
