Team:Paris/Modeling/Workflow Example

Here, we will explain the generic protocol that leads to the modelisation "by characterization", of one of the parts of our system. The choosen example is the activity of the promoter pFliL (that leads to the production of the Fluorescent Protein FP1) in function of the transcription factors FlhDC and FliA . We know that the hexamere FlhD₄C₂ and the protein FliA act both on the promoter pFliL as inducers... how do we evaluate this phenomenon?
As it is illustrated below; starting from molecular reactions (1), we develop a model of our system (2), and then we identify the parameters (3) that needs to be estimated. Essentially, it amounts to estimate Promoters Activities as functions of their Transcription Factors. To do so, we propose constructs (4), protocols (5) and programs (6).

Modelisation	Experiments

Once we got every parameters for every functions involved in our Full System, we have got a "Modular Virtual Lab" of our System that aims to be predictive.