Team:Paris/Modeling/Workflow Example

Here, we explain the generic protocol that leads to modeling "by characterization", of one of the parts of our system. The chosen example is the activity of the promoter pFliL (that leads to the production of the Fluorescent Protein FP1) in function of the transcription factors FlhDC and FliA . We know that the hexamere FlhD₄C₂ and the protein FliA act both on the promoter pFliL as inducers... how do we evaluate this phenomenon?
As it is illustrated below; starting from molecular reactions (1), we develop a model of our system (2), and then we identify the parameters (3) that need to be estimated. Essentially, it amounts to estimate Promoters Activities as functions of their Transcription Factors. To do so, we propose constructs (4), protocols (5) and programs (6).

Modelisation	Experiments

By estimating all parameters of every function involved in our Full System, we achieve a "Modular Virtual Lab" of our System with potential predictive capacity.