Revision as of 00:10, 30 October 2008

Birds Eye View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

Things we did today

Grace

Ran HindIII/BamHI digested J33207 on gel
Extracted from gel
Ligated with HindIII/BamHI digested pRL1383a
Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)

Krystle

BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37^oC

Plate of BB-pRL1383a/K125820 transformants under blue light. Only some of the colonies appear to fluoresce.

Krystle

Inoculated 10ml cultures of LB with
- BB-pRL1383a with sp₁₀₀
- BB-pRL1383a/K125820 with sp₁₀₀
- RP1 with kan₅₀

Grace

Wrote up Materials, Methods and Results for Project Part B on team project page.

Grace

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 14: / Line 14: @@
 ::* Also transformed 25 &mu;l DH5&alpha; MCR with 2 &mu;l BB-1 1025 and 1 &mu;l J33207 each.
-===Inoculate for Tri-Parental===
+===Checked for Transformants===
+:<strong>Krystle</strong>
+:*BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37<sup>o</sup>C
+[[Image:PRL-K125820.jpg|right|thumb|Plate of BB-pRL1383a/K125820 transformants under blue light.  Only some of the colonies appear to fluoresce.]]
+===Inoculate for Tri-Parental Conjugation===
 :<strong>Krystle</strong>