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Team:Caltech/Protocols/H2O2 Production Assay
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# Grow an overnight culture of JI377 transformed with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K137076 K137076] or [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2622 luxR] (negative control) on pSB1A2 in SOC + Amp. | # Grow an overnight culture of JI377 transformed with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K137076 K137076] or [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2622 luxR] (negative control) on pSB1A2 in SOC + Amp. | ||
# In the morning, dilute culture 1:100 into 5 ml SOC +Amp +IPTG in triplicate. | # In the morning, dilute culture 1:100 into 5 ml SOC +Amp +IPTG in triplicate. | ||
| - | # Grow | + | # Grow cultures to an OD600 of ~0.8, then [[Team:Caltech/Protocols/Measuring_H2O2|assay supernatant]] for hydrogen peroxide approximately every 30 minutes. |
# 1hr after reaching an OD600 of ~0.8, induce cultures with 10 nM AHL. | # 1hr after reaching an OD600 of ~0.8, induce cultures with 10 nM AHL. | ||
}} | }} | ||
Latest revision as of 00:13, 30 October 2008
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H2O2 Production Assay
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