Team:Davidson-Missouri Western/New Parts Contributed to the Registry
From 2008.igem.org
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- | '''The Davidson-Missouri Western team contributed 105 new parts to the Registry of Standard Biological Parts. New basic parts contributed included seven novel hybrid promoters, seven promoters and genes to support the Lsr cellular communication system, two improved versions of the pLac promoter, and three new variants of the Lac repressor. All basic parts were sequenced verified. Intermediate parts were constructed by combining new and existing Registry parts. Devices were designed and constructed to function as biological XOR gates and cellular communication components. | + | '''The Davidson-Missouri Western team contributed 105 new parts to the Registry of Standard Biological Parts.''' New basic parts contributed included seven novel hybrid promoters, seven promoters and genes to support the Lsr cellular communication system, two improved versions of the pLac promoter, and three new variants of the Lac repressor. All basic parts were sequenced verified. Intermediate parts were constructed by combining new and existing Registry parts. Devices were designed and constructed to function as biological XOR gates and cellular communication components. |
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Latest revision as of 00:22, 30 October 2008
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The Davidson-Missouri Western team contributed 105 new parts to the Registry of Standard Biological Parts. New basic parts contributed included seven novel hybrid promoters, seven promoters and genes to support the Lsr cellular communication system, two improved versions of the pLac promoter, and three new variants of the Lac repressor. All basic parts were sequenced verified. Intermediate parts were constructed by combining new and existing Registry parts. Devices were designed and constructed to function as biological XOR gates and cellular communication components.
Number | Type | Description | Size | |
---|---|---|---|---|
BBa_K09100 | Composite | Receiver for AHL and Outputs GFP when AHL is present | 1946 bp | |
BBa_K091001 | Coding | LsrR gene | 954 | |
BBa_K091002 | Coding | LsrK gene | 1593 | |
BBa_K091100 | Regulatory | pLac_lux hybrid promoter | 74 | |
BBa_K091101 | Regulatory | pTet_Lac hybrid promoter | 83 | |
BBa_K091104 | Regulatory | Mnt/LacI Hybrid Promoter | 87 | |
BBa_K091105 | Regulatory | Mnt/TetR Hybrid Promoter | 98 | |
BBa_K091106 | Regulatory | LsrA/cI hybrid promoter | 141 | |
BBa_K091107 | Regulatory | Lux-cI hybrid promoter | 57 | |
BBa_K091109 | Coding | LuxS | 516 | |
BBa_K091110 | Regulatory | LacI Promoter | 56 | |
BBa_K091111 | Regulatory | LacIQ promoter | 56 | |
BBa_K091112 | Regulatory | LacIQ1 promoter | 56 | |
BBa_K091114 | Regulatory | LsrAR Promoter | 248 | |
BBa_K091115 | Regulatory | LsrR Promoter | 100 | |
BBa_K091116 | Regulatory | LsrA Promoter | 126 | |
BBa_K091117 | Regulatory | pLas promoter | 126 | |
BBa_K091118 | Intermediate | Codes for LasR and LasR regulated GFP | 1985 | |
BBa_K091119 | Intermediate | LacI protein generator with a pTet promoter | 74 | |
BBa_K091120 | Intermediate | pTet regulated production of LacI protein | 1163 | |
BBa_K091121 | Coding | LacI wild-type gene | 1083 | |
BBa_K091122 | Coding | LacI_I12 protein | 1083 | |
BBa_K091123 | Composite | LacI_I12 protein regulated by pTet | 1163 | |
BBa_K091125 | Composite | GFP generator regulated by pTet | 938 | |
BBa_K091126 | Composite | pTet regulated production of LacI_X86 | 1163 | |
BBa_K091127 | Coding | LacI_I12_X86 | 1083 | |
BBa_K091128 | Composite | pTet regulation of the LacI_I12_X86 protein | 1163 | |
BBa_K091131 | Generator | pLacIQ1 controlled GFP generator | 940 | |
BBa_K091132 | Composite | pMnt/Tet+RBS+LuxI | 767 | |
BBa_K091133 | Composite | pBAD+RBS+TetR | 841 | |
BBa_K091134 | Device | Las Receiver | 2048 | |
BBa_K091136 | Signalling | Las Sender Testing Device | 670 | |
BBa_K091137 | Coding | RBS+Mnt+TT | 468 | |
BBa_K091138 | Composite | RBS+lsrR | 972 | |
BBa_K091139 | Composite | RBS+lsrR+TT | 1109 | |
BBa_K091140 | Composite | plsrA/cI+RBS+luxI | 810 | |
BBa_K091141 | Composite | RBS+Mnt+TT | 468 | |
BBa_K091142 | Intermediate | plsrA/cI+RBS+luxI+RBS+Mnt+TT | 1286 | |
BBa_K091143 | Regulatory | plasR/cI Hybrid Promoter | 164 | |
BBa_K091144 | Composite | pMnt/Tet+RBS+LuxI+RBS+cI+TT | 1671 | |
BBa_K091145 | Composite | pBAD+RBS+TetR+TT | 978 | |
BBa_K091146 | Regulatory | pLas+ Lux- Promoter | 126 | |
BBa_K091147 | Composite | pBAD+RBS+Mnt+TT | 606 | |
BBa_K091148 | Generator | pLas' Promoter Testing Construct A | 1997 | |
BBa_K091149 | Signalling | pLas' Testing Construct B | 1997 | |
BBa_K091150 | Signalling | pLas' Testing Construct C | 2804 | |
BBa_K091151 | Signalling | pLas' Testing Construct D | 2761 | |
BBa_K091152 | Signalling | pLas' Testing Construct D | 2804 | |
BBa_K091153 | Signalling | Standard RBS with LuxS | 534 | |
BBa_K091154 | Composite | RBS+luxI+RBS+Mnt+TT | 1137 | |
BBa_K091155 | Intermediate | plasR/cI+RBS+luxI+RBS+Mnt+TT | 1309 | |
BBa_K091156 | Regulatory | pLux | 55 | |
BBa_K091157 | Regulatory | pLux+ Las- Promoter | 55 | |
BBa_K091158 | Composite | RBS+lsrK | 1611 | |
BBa_K091159 | Composite | pBAD+RBS+lsrK | 1749 | |
BBa_K091160 | Composite | pMnt/Tet-RBS-GFP-TT | 982 | |
BBa_K091161 | Signalling | RBS+LasI+RBS+Mnt+TT | 1103 | |
BBa_K091162 | Signalling | Promoter+RBS+LasI+RBS+Mnt+TT | 1168 | |
BBa_K091163 | Signalling | RBS+LuxS+RBS+Mnt+TT | 1010 | |
BBa_K091164 | Signalling | Promoter+RBS+LuxS+RBS+Mnt+TT | 1075 | |
BBa_K091165 | Reporter | pLux/cI+RBS+GFP+TT | 941 | |
BBa_K091166 | Signalling | pBAD-RBS-luxR | 937 | |
BBa_K091167 | Signalling | RBS+LuxI+TT | 798 | |
BBa_K091168 | Composite | pLac/Mnt+RBS+LuxS+RBS+cI+TT | 1533 | |
BBa_K091169 | Composite | plasR/cI+E0240(GFP) | 1048 | |
BBa_K091170 | Composite | plsrA/cI+E0240(GFP) | 1025 | |
BBa_K091171 | Composite | pBAD+RBS+cI+TT | 1034 | |
BBa_K091172 | Intermediate | lsr system supporting genes | 2866 | |
BBa_K091173 | Device | pLsr/cI+RBS+luxI+RBS+Mnt+TT+pTet/Mnt+RBS+luxI+RBS+cI+TT | 2965 | |
BBa_K091174 | Device | pBAD-RBS-TetR-TT-pBAD-RBS-LsrK-RBS-LsrR-TT | 3852 | |
BBa_K091175 | Composite | pBAD-RBS-tetR-TT-pMnt/tetR-RBS-GFP-TT | 1968 | |
BBa_K091176 | Composite | pBAD-RBS-Mnt-TT-pMnt/tetR-RBS-GFP-TT | 1596 | |
BBa_K091177 | Composite | pMnt/tetR-RBS-GFP-TT-pBAD-RBS-tetR-TT | 1968 | |
BBa_K091178 | Composite | pMnt/tetR-RBS-GFP-TT-pBAD-RBS-Mnt-TT | 1596 | |
BBa_K091179 | Signalling | LuxR receiver and pBAD promoter | 1074 | |
BBa_K091180 | Composite | pLac-RBS-LasR-TT | 999 | |
BBa_K091181 | Composite | pBad-RBS-tetR-TT-placI/pI-RBS-lasR-TT | 1985 | |
BBa_K091182 | Composite | pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT | 2988 | |
BBa_K091183 | Signalling | pBAD+RBS+LuxR+RBS+cI lam+TT | 1841 | |
BBa_K091184 | Regulatory | pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT | 2616 | |
BBa_K091185 | Composite | LacI_I12+TT | 1220 | |
BBa_K091186 | Composite | pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | 2528 | |
BBa_K091188 | Composite | pMnt/lac+RBS+lasI+RBS+cI+TT | 1626 | |
BBa_K091190 | Composite | plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT | 2802 | |
BBa_K091191 | Composite | pBAD+RBS+luxS+TT | 809 | |
BBa_K091192 | Composite | pBAD+RBS+lasI+TT | 807 | |
BBa_K091194 | Reporter | Promoter testing construct | 2023 | |
BBa_K091195 | Reporter | Promoter testing construct | 1983 | |
BBa_K091196 | Reporter | Promoter testing construct | 2790 | |
BBa_K091197 | Intermediate | plsrAcI testing construct with GFP | 3899 | |
BBa_K091198 | Intermediate | plasRcI testing construct | 2055 | |
BBa_K091199 | Intermediate | plasR/cI overall testing construct | 3097 | |
BBa_K091200 | Intermediate | plsrA/cI testing construct with lambda cI repression | 2067 | |
BBa_K091201 | Intermediate | plsrAcI overall testing construct | 4941 | |
BBa_K091202 | Intermediate | plasRcI testing construct with lambda cI repression | 2090 | |
BBa_K091203 | Device | pBAD-RBS-LuxR-RBS-LacI(I12)-TT | 2183 | |
BBa_K091204 | Coding | Prom-LuxR-TT | 979 | |
BBa_K091205 | Coding | Prom-LasR-TT | 979 | |
BBa_K091206 | Coding | Prom-LuxR-TT-Prom-LasR-TT | 1966 | |
BBa_S03967 | Intermediate | RBS-LasI | 627 | |
BBa_S03968 | Intermediate | RBS-LuxS | 534 | |
BBa_S03969 | Intermediate | pMnt/Lac-RBS-GFP-TT | 971 | |
BBa_S03979 | Intermediate | RBS-LuxS-RBS-cI-TT | 1438 | |
BBa_S03980 | Intermediate | pBAD-RBS-LacI-TT | 1446 | |
BBa_S04058 | Intermediate | RBS-LacI12-TT | 1238 |
Safety considerations: To the best of our knowledge, the new parts above pose no threats to researcher, public, or environmental safety. Student researchers are instructed by faculty mentors in the use of best practices in laboratory procedures when handling bacteria and reagents.
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