Team:BrownTwo/Limiter/altdesign

From 2008.igem.org

(Difference between revisions)
Line 9: Line 9:
*Smolke’s gal2 knockout scheme for using the GAL1 promoter
*Smolke’s gal2 knockout scheme for using the GAL1 promoter
*Finding additional inducible/repressible promoters to use in the system
*Finding additional inducible/repressible promoters to use in the system
 +
 +
*Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)

Revision as of 00:58, 30 October 2008



Alternative construction and design possiblities

  • up-limiter
  • dual limiter
  • limiter device as a regulatory bridge between two pathways
  • killswitch
  • Smolke’s gal2 knockout scheme for using the GAL1 promoter
  • Finding additional inducible/repressible promoters to use in the system
  • Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
Retrieved from "http://2008.igem.org/Team:BrownTwo/Limiter/altdesign"