Team:Hawaii/Notebook/2008-10-28

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:*BB-pRL1383a/K125810 produced a total of 27 transformants of varying size after incubation for 20 hours at 37<sup>o</sup>C
:*BB-pRL1383a/K125810 produced a total of 27 transformants of varying size after incubation for 20 hours at 37<sup>o</sup>C
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[[Image:PRL-K125820.jpg|right|thumb|Plate of BB-pRL1383a/K125810 transformants under blue light.  Only some of the colonies appear to fluoresce.]]
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[[Image:PRL-K125820.jpg|right|thumb|Plate of BB-pRL1383a/K125810 transformants under blue light.  Only some of the colonies appear to fluoresce and the smaller colonies do not show well in the picture.]]
===Inoculate for Tri-Parental Conjugation===
===Inoculate for Tri-Parental Conjugation===

Revision as of 01:20, 30 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Antibiotic test

Grace
  • Ran HindIII/BamHI digested J33207 on gel
  • Extracted from gel
  • Ligated with HindIII/BamHI digested pRL1383a
  • Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
  • Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.

Checked for Transformants

Krystle
  • BB-pRL1383a/K125810 produced a total of 27 transformants of varying size after incubation for 20 hours at 37oC
Plate of BB-pRL1383a/K125810 transformants under blue light. Only some of the colonies appear to fluoresce and the smaller colonies do not show well in the picture.

Inoculate for Tri-Parental Conjugation

Krystle
  • Inoculated 10ml cultures of LB with
    • BB-pRL1383a with sp100
    • BB-pRL1383a/K125810 with sp100
    • RP1 with kan50

Drylab Work

Project page

Grace
  • Wrote up Materials, Methods and Results for Project Part B on team project page.

Sequence analysis

Grace
  • Same G->C transversion in CAP binding site for BBpRL-1 sequence.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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