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User contributions
From 2008.igem.org
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- 16:37, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/4 August 2008
- 20:46, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/28 July 2008 (New page: == Team Sorbitol == Harvested the DNA from these cultures and digested - however they were not the right size so will reperform the ligation)
- 20:45, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/27 July 2008 (New page: == Team Sorbitol == Got several colonies from the transformation - grew them up in liquid culture.)
- 20:44, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/26 July 2008 (New page: == Team Sorbitol == Transformed the ligations into chemically competent DH5a and allowed to grow overnight)
- 20:44, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/25 July 2008 (New page: == Team Sorbitol == Digested the ''srlD'' and pET28a with BamHI and HindIII Extracted the digestion and performed a ligation which ran overnight at 4C)
- 20:42, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/23 July 2008
- 20:41, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/23 July 2008 (New page: == Team Sorbitol == Sucessfully cloned out the ''srl'' operon)
- 20:40, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/18 July 2008 (New page: == Team Sorbitol == Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD. They were then stored at -80. Primers were then designed and ordered to insert '...)
- 20:38, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/17 July 2008 (New page: == Team Sorbitol == Grew up 2mL cultures of the successful transformations from 7/15. Also designed several primers that will be used for biobricking ''srlD'' We ordered these primers a...)
- 20:37, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/15 July 2008 (New page: == Team Sorbitol == All the transformations worked correctly)
- 20:35, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/14 July 2008 (New page: == Team Sorbitol == Met with Dr. Pfleger and learned the specifics of biobricking. Designed a procedure to biobrick our genes. Following Weibel Lab protocols transformed pBAD30-srlD int...)
- 20:31, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/11 July 2008 (New page: == Team Sorbitol == Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use. Harvested the DNA from the pBAD30, PBAD30-srlD, and pBAD18 using a miniprep kit. ...)
- 20:29, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/10 July 2008
- 20:28, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/10 July 2008
- 20:25, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/10 July 2008 (New page: == Team Sorbitol ==)
- 20:24, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/9 July 2008 (New page: == Team Sorbitol == The sequencing was cleaned with Agencourt CleanSEQ dye-Terminator removal). It was then taken to the UW-biotech center for sequencing. Attended the Wisconsin iGEM te...)
- 20:23, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/8 July 2008 (New page: == Team Sorbitol == Harvested the DNA from the overnight colonies. Used this DNA in a PCR sequencing reaction. The product was wrapped in foil (to keep out the light) and stored at 4C o...)
- 20:22, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/7 July 2008 (New page: == Team Sorbitol == The 10 colonies were harvested with a Qiagen Miniprep Kit and the DNA was digested with Xba1 and HindIII for 1 hour at 37C. The digestion was then run on an agarose g...)
- 20:19, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/6 July 2008 (New page: == Team Sorbitol == Took out the transformed plates - all grew! Started liquid cultures of 10 colonies to verify the transformation was correct)
- 20:18, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/28 May 2008
- 20:18, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/3 July 2008 (New page: == Team Sorbitol == Created SOC media for transformations. Transformed each of the ligations (as well as controls) into our chemically competent stocks of DH5a. To do this we followed th...)
- 20:16, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/2 July 2008
- 20:16, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/2 July 2008
- 20:15, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/2 July 2008 (New page: == Team Sorbitol == The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before. We were able to extract enough plasmid DNA to perform a ligation. We then set ...)
- 20:13, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/1 July 2008 (New page: == Team Sorbitol == The overnight PCR reaction failed. Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before. The digestion was veri...)
- 20:10, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/30 June 2008 (New page: == Team Sorbitol == Repeated the successful PCR reaction from before. This time we obtained successful band for both ''srlD'' 700bp and the ''srl'' operon 2700bp These products were puri...)
- 20:07, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/29 June 2008 (New page: == Team Sorbitol == Began a 10mL culture of DH5a with pBAD30 in order to maximize our amount of DNA obtained after a digestion.)
- 20:06, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/27 June 2008
- 20:03, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/26 June 2008
- 20:00, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/24 June 2008
- 19:59, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/25 June 2008
- 19:57, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/24 June 2008
- 19:56, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/23 June 2008
- 19:54, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/19 June 2008
- 19:50, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/18 June 2008
- 19:48, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/17 June 2008
- 19:47, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/17 June 2008
- 19:45, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/16 June 2008
- 19:43, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/15 June 2008 (New page: == Team Sorbitol == Started culturse of DH5a containing both pBAD30 and pBAD18)
- 19:37, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/12 June 2008 (New page: == Team Sorbitol == We designed and ordred primers for cloning ''srlD'' and the ''srl''operon out of E. coli MG1655. These primers will also add an xba1 site as well as HindIII site to...)
- 19:32, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/11 June 2008 (New page: == Team Sorbitol == Attended the Wisconsin iGEM team meeting. Performed a growth curve of E. coli MG1655 in the three formulas created yesterday. We also researched more specific infor...)
- 19:30, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/10 June 2008 (New page: == Team Sorbitol == Created stocks of M9 Minimal media We then created 3 solutions: M9 + Glucose M9 + acetate M9 + no other carbon source With these we will be able to test the abili...)
- 19:28, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/9 June 2008 (New page: '''Team Sorbitol:'''<br> Planned to clone out ''srlD'' as well as the ''srl'' operon from E. coli MG1655 (K12 parent strain). ''srlD'' codes for the sorbitol-6-phosphate dehydrogenase '...)
- 19:23, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/5 June 2008 (New page: Another Wisconsin Team meeting took place. '''Team Sorbitol:'''<br> Began experimenting with the modeling program to determine if our planned knockouts would work or not.)
- 19:22, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/4 June 2008 (New page: '''Team Sorbitol:'''<br> Learned how to do pathway modeling in E. Coli from Dr. Reed. We will use this in the future to model different knock outs to up-regulate the most sorbitol as pos...)
- 19:20, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/3 June 2008 (New page: '''Team Sorbitol:'''<br> Began punching out biobricks from the iGEM registry. '''Team Lignin:'''<br>)
- 19:19, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/2 June 2008 (New page: '''Team Wisconsin:'''<br> Both teams discussed possible problems they may encounter and received possible solutions from our faculty sponsors. '''Team Sorbitol:'''<br> We began dividing...)
- 19:13, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/29 May 2008 (New page: '''Team Wisconsin:'''<br> Each team wrote out a one page summary and presented it to our advisors. We began contacting companies and organizations for support.)
- 19:12, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/28 May 2008 (New page: '''Team Wisconsin'''<br> Prepared chemically competent stocks of E.coli top10 and DH5a. These cells will be used for future transformations.)
- 19:03, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/27 May 2008 (New page: '''Wisconsin iGEM Team Begins'''<br> The Wisconsin teams is formed and divided into two sub-teams: '''Team Sorbitol:'''<br> - Will attempt to produce large amounts of sorbitl using E. co...)
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