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User contributions
From 2008.igem.org
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- 08:46, 29 October 2008 (diff | hist) Team:Cambridge/Voltage
- 17:37, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/Output (New page: =Setup= Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise. *Cells containing b...)
- 17:36, 28 October 2008 (diff | hist) Team:Cambridge/Voltage
- 17:36, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Extracted Parts (New page: {{Cambridge08a}} <html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:x="urn:schemas-microsoft-com:office:excel" xmlns="http://www.w3.org/TR/REC-html40"> <head> <meta http-equi...)
- 17:36, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/GluR0 Manipulation (New page: =GluR0 - [http://partsregistry.org/Part:BBa_K090001 K090001]= GluR0 has been well characterised in Synechocystis and the amino acid sequence fully documented. The sequence was reverse tran...)
- 17:36, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/BioBrick Manipulation (New page: =OsmY RBS= OsmY was PCRed out of the biobricks which contained it - BBa_J45996 (A) , BBa_J45995 (B) and BBa_J45250 (C). The primers were designed to have the BioBrick prefix and suffix, b...)
- 17:35, 28 October 2008 (diff | hist) N File:MutantPotassium.xls (top)
- 17:34, 28 October 2008 (diff | hist) IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations (→Results)
- 17:34, 28 October 2008 (diff | hist) IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations (→Results)
- 17:34, 28 October 2008 (diff | hist) IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations (→Results)
- 17:34, 28 October 2008 (diff | hist) IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations (→Results)
- 17:34, 28 October 2008 (diff | hist) N File:KJW1242.JPG (top)
- 17:33, 28 October 2008 (diff | hist) N File:KJW0045.JPG (top)
- 17:33, 28 October 2008 (diff | hist) N File:KB30166.JPG (top)
- 17:33, 28 October 2008 (diff | hist) N File:KJW3314.JPG (top)
- 17:33, 28 October 2008 (diff | hist) N File:KBW25113.JPG (top)
- 17:33, 28 October 2008 (diff | hist) IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations
- 17:32, 28 October 2008 (diff | hist) N File:Kallstrains2.JPG (top)
- 17:32, 28 October 2008 (diff | hist) N File:Kallstrains.JPG (top)
- 17:31, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations (New page: =Method= *Five different mutant strains were selected for investigation, based on their growth data and theoretical prediction about potassium sequestration capability. [[IGEM:Cambridge/...)
- 17:30, 28 October 2008 (diff | hist) N File:MutantGrowthData.xls (top)
- 17:29, 28 October 2008 (diff | hist) N File:RelGrowth200mmK.JPG (top)
- 17:29, 28 October 2008 (diff | hist) N File:RelGrowth50mmK.JPG (top)
- 17:29, 28 October 2008 (diff | hist) N File:RelGrowth1mmK.JPG (top)
- 17:28, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/K+ Growth (New page: ==Method== *For each of the 9 mutants, 3 [K+] of growth medium were examined: 1mM, 50mM & 200mM '''Composition of Each Cuvette:''' * 100ul cells ...)
- 17:26, 28 October 2008 (diff | hist) N File:Voltage button.gif (top)
- 17:26, 28 October 2008 (diff | hist) Team:Cambridge/Voltage
- 17:25, 28 October 2008 (diff | hist) Team:Cambridge
- 17:23, 28 October 2008 (diff | hist) N File:0D600 curve.JPG (top)
- 17:23, 28 October 2008 (diff | hist) N File:High concs.JPG (top)
- 17:22, 28 October 2008 (diff | hist) N File:Low concs.JPG (top)
- 17:19, 28 October 2008 (diff | hist) N File:Glur0plasmid.JPG (top)
- 17:19, 28 October 2008 (diff | hist) N File:Psb4c5 osmy b0030 kdp.JPG (top)
- 17:19, 28 October 2008 (diff | hist) N File:Psb4c5 osmy b0030.JPG (top)
- 17:19, 28 October 2008 (diff | hist) N File:Osmy b0032 pcr prod.JPG (top)
- 17:19, 28 October 2008 (diff | hist) N File:Osmy b0031 pcr prod.JPG (top)
- 17:18, 28 October 2008 (diff | hist) N File:Osmy b0030 pcr prod.JPG (top)
- 17:17, 28 October 2008 (diff | hist) IGEM:Cambridge/2008/Notebook/Voltage/Gene Design (→Integration into Vector)
- 17:17, 28 October 2008 (diff | hist) N File:Psb4c5 kdp.JPG (top)
- 17:17, 28 October 2008 (diff | hist) N File:Psb4c5.JPG (top)
- 17:16, 28 October 2008 (diff | hist) N File:Kdp pcr prod.JPG (top)
- 17:16, 28 October 2008 (diff | hist) IGEM:Cambridge/2008/Notebook/Voltage/Gene Design (→Gene Selection)
- 17:14, 28 October 2008 (diff | hist) N File:Kdpfull.JPG (top)
- 17:12, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/Mutant Strains (New page: Mutant strains were required to identify and remove any interference from E. Coli's natural K+ homeostatic mechanisms. The following mutants were ordered from [http://cgsc.biology.yale.edu...)
- 17:12, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/OD600 Calibration (New page: =Method= *An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.) *Dilutions were plated out at 10<sup>-5</s...)
- 17:12, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/Flame Photometer Calibration (New page: =Method= =Results= Calibration for low concentrations: 800px Calibration for high concentrations: 800px)
- 17:11, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage (New page: <hr width="826px"> <div id="about"> {{Cambridge08a}} </div> <hr width="826px"> __NOTOC__ {|{{table}} width="820" |- |style="background-color: #000000;" align="center"| <!-- ## START calend...) (top)
- 17:10, 28 October 2008 (diff | hist) N IGEM:Cambridge/2008/Notebook/Voltage/Gene Design (New page: =KdpF-C Biobrick= ==Gene Selection== *Kdp is a well documented P-Type K+ ATPase found naturally in E.coli, used to actively pump ions into the cell. *It consists of a 6-gene operon: F,A,B...)
- 17:10, 28 October 2008 (diff | hist) N Team:Cambridge/Voltage/Technical Information (New page: ==Technical Information== Gene Design [[IGEM:Cambridge/2008/Notebook/Voltage/Flame Photometer Calibration|Flame Photometer Calibratio...) (top)
- 20:37, 1 September 2008 (diff | hist) N User:Ellis (New page: Ellis O'Neill is a 3rd year undergraduate in Cambridge University ==Contact Info== *Ellis O'Neill *Corpus Christi College *Cambridge *UK. E-mail at: eco23@cam.ac.uk Works in the Gene...) (top)
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