Team:BrownTwo/Limiter/altdesign

Examples of Additional Control Systems

The synthetic transcriptional control system that we have added to the registry is a powerful tool, as its elements could be put together in an enormous variety of ways. These are some possibilities that we have drawn up:

• up-limiter - upregulates a gene when the expression falls below a certain level
• middle-manager- a combination limiter and up-limiter, it keeps a gene's expression between two threshold levels
• dual limiter - regulates two genes simultaneously based on the induction of one.
• limiter device as a regulatory bridge between two pathways- the induction of one gene causes threshold regulation of another
• directed evolution for transcriptional phenotypes - express a cell-death gene if the level of induction a given gene is above or below a threshold level