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Team:iHKU/Notebook
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Wet lab log book for the biobrick subgroup
(Many Thanks to MIT team for setting a good example for us ^_^)
- Click on any day below to see what wet-lab procedures were conducted.
- log book summary
0703 done: punched: and transformed. BBa_Q04510 BBa_R0062 BBa_R0011 BBa_I732096
Planning and summary section for the biobrick subgroup
here is the Biobrick subgroup progress,
- Roy has got these following biobircks:
[http://partsregistry.org/Part:BBa_E0240 Part:BBa_E0240] Colony standard measurement for promotor
p1010 psb3K3
- parts already obtained by peter：
[http://partsregistry.org/Part:BBa_F2622 Part:BBa_F2622] Colony
[http://partsregistry.org/Part:BBa_T9002 Part:BBa_T9002] Punch
[http://partsregistry.org/Part:BBa_I731007 Part:BBa_I731007] Punch
[http://partsregistry.org/Part:BBa_B0030 Part:BBa_B0030] Punch
S17 strain colony ready for ccdB
- parts we further need to get
[http://partsregistry.org/Part:BBa_Q04510 Part:BBa_Q04510] 1013 7A Transf
[http://partsregistry.org/Part:BBa_R0062 Part:BBa_R0062] 1000 4G Transf
[http://partsregistry.org/Part:BBa_R0011 Part:BBa_R0011] 1000 4b Transf
[http://partsregistry.org/Part:BBa_I732096 BBa_I732096] 1014 3G Transf reporter containing the standard GFP and LacZa
- parts that may be useful
http://partsregistry.org/Part:BBa_B0031
http://partsregistry.org/Part:BBa_B0032
http://partsregistry.org/Part:BBa_B0033
http://partsregistry.org/Part:BBa_B0034
- parts that we need to build:
1 [http://partsregistry.org/Part:BBa_K094001 Part:BBa_K094001]CheZ assumed without rbs
2 [http://partsregistry.org/Part:BBa_K094002 Part:BBa_K094002]LuxRI assumed with rbs and terminator
- composite we need to construct:
1[http://partsregistry.org/Part:BBa_K094003 plaLuxRpluxCheZGFPLva] BBa_F2622 BBa_B0030 BBa_K094001 BBa_B0030 BBa_E0022 BBa_B0015 （something wrong with GFPLva and i will change them later）
2[http://partsregistry.org/Part:BBa_K094004 plaLuxRpluxCIpldCheZGFPLva] BBa_F2622 BBa_Q04510 BBa_B0030 BBa_K094001 BBa_E0430
3[http://partsregistry.org/Part:BBa_K094005 plaLuxRpluxLacZ] BBa_F2622 BBa_B0030 BBa_I714901 BBa_B0015
4[http://partsregistry.org/Part:BBa_K094006 plaLuxRpluxCIpldLacZ] BBa_F2622 BBa_Q04510 BBa_B0030 BBa_I714901 BBa_B0015
5[http://partsregistry.org/Part:BBa_K094007 plaLuxRIpluxCheZGFPLva] BBa_R0011 BBa_K094002 BBa_R0062 BBa_B0030 BBa_K094001 BBa_E0430
6 [http://partsregistry.org/Part:BBa_K094008 first]
7 [http://partsregistry.org/Part:BBa_K094009 Part:BBa_K094009]
Notebook
If it were not igem, we wouldn’t have a chance to work on the project of our own. There are many student helpers with very interesting projects in the lab, and definitly they would gain an experience in genetic engineering. However they have much less freedom—although their inteligence enable them to choose the best way to achieve the goal, they seldom choose the goal by themselves. But we can. It is not because we are smarter, but because of the nature of the competition, which requires originality right from the choice of goal. Just imagine how it feels when you speak aloud your wildest ideas from bacti-voice recorder to E-coli insulin injector and the professor sitting there listening and trying to help you to find a way out.
If it were not igem, we wouldn’t have a chance to collabrate with the Physics, Chemistry, and Engineer students. Now it is not only possible but a must to be diverse. In the brain storming session, a “mission impossible” raised by an Engineering student might be solved with ease by a biological student; a common sense in physics might provide inspiration to a biochemical student getting lost in details... When it comes to modelling, it is more efficient to invite a student specializing in matlab rather than borrowing a book and learn programing from ABC by yourself. Thanks to iGEM, the intelligence from science, engineering and medicine faculty all add up to make a difference.
If it were not igem, we wouldn’t have a chance to work as a team. Our working enviroment is always lively and refreshing because we are a group sharing a common goal—a grand prize. Thanks to the common goal, whoever has a problem, the whole team will try to figure out a solution; whoever has an new finding, everyone will rush at the situation trying to give an intepretation, and whenever one read an interesting paper, the whole group will be informed of it in a short time.
Thanks to Dr Huang, iGEM has become part of our life rather than another big game irrelated to us--At a time when dream is a luxury, we are lucky ones whose dream has been nurtured. And it is only a beginning...
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