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Team:KULeuven/Tools
From 2008.igem.org
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Contents |
Templates
General
Example Usage:
{{:Team:KULeuven/Tools/Navigation_Bar}}
The navigation bar used throughout the wiki.
New Day
Example Usage:
{{#calendar: query=preload=:Team:Kuleuven/Tools/New_Day}}
The day template that is preloaded into a notebook day. The result can be seen in the Notebook section, when you
click on an unmodified day, you'll get the edit screen, prefilled with the contents of the New_Day template.
Lab Templates
PCR sheet
Example Usage:
{{:Team:KULeuven/Tools/PCR
|tanneal=55
|telong=2'30"
|ncycles=30
|buffer=5x 10μL
|mgcl=10μM 0μL
|dntp=10μM 1μL
|fwprimername=6 LacZ-Fw
|fwprimerconc=10μM 2.5μL
|rvprimername=7 LacZ-Rv
|rvprimerconc=10μM 2.5μL
|water=34μL
|polymerase=Taq 0.5μL
|dna=0.5μL of E. coli DNA extract
|size=952bp
|success=YES/NO
|image=pcrproducts_GE.jpg
|lane=3+4
}}
Result:
| PCR: | |||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Digestion sheet
Example Usage:
{{:Team:KULeuven/Tools/Digestion
|nr=D12
|product=Cre ORF
|matrix=pJ61047
|enz1=Pst1
|enz2=Xba1
|size=1063bp
|desc=Cre ORF extracted for further usage
}}
Result:
| Digestion Products | ||||||
|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description |
| D12 | Cre ORF | pJ61047 | XbaI | PstI | 1063bp | Cre ORF extracted for further usage |
Ligation sheet
Example Usage:
{{:Team:KULeuven/Tools/Ligation
|nr=L21
|insert=D12 Cre ORF
|insert_vol=6µL
|vector=D1 pJ23100
|vector_vol=2µL
|comm=construction of BioBrick: Cre-recombinase under control of strong promotor
|ncol=30
}}
Result
| Ligations | ||||||
|---|---|---|---|---|---|---|
| Number | Insert | Insert Volume (µL) | Vector | Vector Volume (µL) | Comments | Number of colonies |
| L21 | D12 Cre ORF | 6µL | D1 pJ23100 | 2µL | construction of BioBrick: Cre-recombinase under control of strong promotor | 30 |
96-Well Plate
Example Usage:
{{:Team:KULeuven/Tools/96-well
|title=Induced Response
|A1=LB + 0µM IPTG
|A2=LB + 0µM IPTG
|A3=LB + 5µM IPTG
|A4=LB + 5µM IPTG
|A5=LB + 10µM IPTG
|A6=LB + 10µM IPTG
|A7=etc
|B1=LB 2 + 0µM IPTG
|C1=LB 3 + 0µM IPTG
|D1=LB 4 + 0µM IPTG
|E1=LB 5 + 0µM IPTG
|F1=etc
}}
Result
| 96-Well Plate: Induced Response | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | LB + 0µM IPTG | LB + 0µM IPTG | LB + 5µM IPTG | LB + 5µM IPTG | LB + 10µM IPTG | LB + 10µM IPTG | etc | / | / | / | / | / |
| B | LB 2 + 0µM IPTG | / | / | / | / | / | / | / | / | / | / | / |
| C | LB 3 + 0µM IPTG | / | / | / | / | / | / | / | / | / | / | / |
| D | LB 4 + 0µM IPTG | / | / | / | / | / | / | / | / | / | / | / |
| E | LB 5 + 0µM IPTG | / | / | / | / | / | / | / | / | / | / | / |
| F | etc | / | / | / | / | / | / | / | / | / | / | / |
| G | / | / | / | / | / | / | / | / | / | / | / | / |
| H | / | / | / | / | / | / | / | / | / | / | / | / |
Collection Templates
Primer Collection
Primer Collection Wiki Page
To start the primer collection wiki page, simply create a new page and add following lines:
{|width="960" cellspacing="1"
! style="background:#003E81; color: white" colspan="5" | Table of Oligos
|-style="background:#66FFCC" align="center"
| width="40pt" | Number
| width="80pt" | Name
| width="500pt" | Sequence
| width="30pt" | Length
| width="300pt" | Comments
...
|}
Where you see the dots the template codeline will be added to.
Example Usage
Result
