"
Virginia/8 July 2008
From 2008.igem.org
Goals
- Prepare overnight broth of single successful colony of Promoter + RBS transformation
- Plate Screening plasmid (psb1a10) when it arrives from iGem
- Try again with ligation of RFP and GFP enzyme cut DNA
- Transform this ligation and pray that it grows in the incubator
- Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
- Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon
Notes
- Maxiprepped bba_B0015 and bba_B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA
