Minnesota/10 July 2008
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Revision as of 16:04, 10 July 2008 by Emartin9808 (Talk | contribs)
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1. Dephosphorylation: Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product.
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NOTE: Total volume in dephosphorylation = 56.6uL |