Minnesota/10 July 2008

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Revision as of 16:04, 10 July 2008 by Emartin9808 (Talk | contribs)
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1. Dephosphorylation: Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product.
Gene 10x Buffer H20 DNA Phosphotase
Base Vector 5.6uL -- 50.0uL 1.0uL
NOTE: Total volume in dephosphorylation = 56.6uL