Team:Johns Hopkins/Notebook/GROUP 6: Vectors

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GROUP 6: Vectors

 Status report by ____
 Vector transformed into bacteria strain DB3.1 Y/N
 Permanent culture made in the Boeke lab for future reference Y/N
 Selectable marker for this vector
 Medium made and tested Y/N  (link)
 DNA preps made Y/N
 DNA preps tested by RE digest  - (link)
 DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) – put data as 
   Table on moodle.  
 Sample format/data follows:

 Amount transformed		cfu/micGm in DB3.1		cfu/micGm in JM109
 0.1 ng					5 * 10e7			<2 * 10e2

 Preparative digests ready for use are located – where?

 [http://www.jhu.edu/iGEM/GroupOther:Other/2008-7-24.Vectors%20(CAM,AMP,KAN3,KAN4)%20after%20Digest.Tejas.html Vectors (CAM,AMP,KAN3,KAN4) after Digest]