"
Team:BCCS-Bristol/Calendar-Notebook/1 July 2008
From 2008.igem.org
This is a page for any solutions
| Media for swimming agar assay plates | 0.3% 400ml | 0.15% 150ml | 0.5% 150ml |
| Bactotryptone | 4g | 1.5g | 1.5g |
| NaCl | 2g | 0.75g | 0.75g |
| BactoAgar | 1.2g | 0.225g | 0.75g |
in addition did a streak out for master colonies of MG1655 in LB from table below overnight at 37oC
| 10ml LB | ' |
| Yeast Extract | 0.05 |
| Tryptone | 0.1 |
| Nacl | 0.1 |
| LB Broth 100ml | weight (g) |
| Yeast Extract | 0.5 |
| Tryptone | 1 |
| NaCl | 1 |
SOC medium in preparation for Biobrick transformation this consisted of
| Trypton | 2g |
| Yeast Extract | 0.5g |
| NaCl | 0.05g |
in 95ml of water, then autoclaved. after this add this:
| MgCl2 | 0.5ml 2M solution |
| Glucose | 2ml 1M |
then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
Swimming Medium
| In 100ml of water | weight (g) |
| Potassium Phosphate | 0.1742 |
| NaCl | 0.392 |
| Glucose | 0.18016 |
| EDTA | 0.003722 |
| Tween -20 aka polysorbate 20 | 0.2 MICRO litres |
