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Things we did today

Wetlab work

Reconstruction of BB-pRL1383a

EtBr stained 1% agarose gel ran at 72V for ... Thirty-five microliters of RE digested BB-pRL1383a and fifty microliters of RE digested J33207 were loaded.

Grace

PCR amplification of J33207

ExoSAP'd PCR product

RE digested of J33207 and BB-pRL1383a (w/ GFP)
Ran digests on a 1% agarose gel
Extracted from bands from gel
Ligated J33207 w/ BB-pRL1383a
Transformed into DH5α

Drylab Work

Name of Task

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

Discussion

DH5α does not carry lacI^q; therefore, IPTG is not neccessary to induce the lac promoter.
IPTG is necessary for induction in DB3.1 cells.

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

Team:Hawaii/Notebook/2008-08-15