Edinburgh/Notebook/Plates

From 2008.igem.org

Revision as of 10:23, 21 August 2008 by Andhi (Talk | contribs)

Edinburgh iGem 2008

 

Plates 1 and 2: transformation with a BioBrick from the Registry stock: failed. (18.06.08)
Plate 3: L1 (pSB1A2+dxs) transformation, 100μl. (27.06.08)
Plate 4: L2 (pSB1A2+appY) transformation, 100μl. (27.06.08) - Failed
Plate 5: L3 (BABEL1+glgC) transformation, 100μl. (27.06.08)
Plate 6: L4 (BABEL2+glgC) transformation, 100μl. (27.06.08)
Plate 7: L1 (pSB1A2+dxs) transformation, 900μl. (27.06.08)
Plate 8: L2 (pSB1A2+appY) transformation, 900μl. (27.06.08) - Failed
Plate 9: L3 (BABEL1+glgC) transformation, 900μl. (27.06.08)
Plate 10: L4 (BABEL2+glgC) transformation, 900μl. (27.06.08)
Plate 11: possible pSB1A2+dxs transformants. (28.06.08)
Plate 12: possible Babel1+glgC transformants. (28.06.08)
Plate 13: possible Babel2+glgC transformants. (28.06.08)
Plate 14: more subcultures from plates 5 and 6. (29.06.08)
Plates 15 and 16: retransformation of religated L2. Failed again. (03.07.08)
Plate 17: Pantoea ananatis for crt PCR reactions. (05.07.08)
Plates 18 and 19: L5 (pSB1A2+appY) transformation, 100μl and 900μl. (05.07.08)
Plates 20: subculture from plate 19. (06.07.08

  • Plate 21:* subculturees from 18 and 19.
  • Plates 22 and 23:* L6 transformation. No colonies.
  • Plates 24 and 25:* L7 transformation.
  • Plates 26 and 27:* subcultures from plates 24 and 25 (L7).
  • Plates 28 and 29:* _Cellulomonas_ from initial stock.
  • Plates 30 and 31:* possible pSB1A2\-_crtB_ transformants.
  • Plates 32 and 33:* possible pSB1A2\-_crtI_(mutant) transformants.
  • Plate 34:* possible rbs\+_dxs_ clones from Nimisha's plates.
  • Plate 35:* patches of possible pSB1A2\-_crtB_ transformants from Plate 30.
  • Plate 36:* patches of possible pSB1A2\-_crtI_(mutant) transformants from Plates 32 and 33.
  • Plate 37:* subcultuure of _Cellulomonas fimi_ on NA with filter paper (cellulose)
  • Plates 38 and 39:* L15 transformation (rbs+dxs PCR product with Edinbrick1)
  • Plates 40 and 41:* L13 transformation (glgC M19 PCR produce to mutate site 1)
  • Plates 42, 44 and 45:* Patches of possible glgC M19 transformants - subcultures from plate 41
  • Plate 43:* Patches of possible dxs+rbs transformants - subculture from plate 38 or 39
  • Plates 46~47:* L16 transformation (appY+rbs PCR product with Edinbrick 1) (18/07/08)
  • Plates 48~50:* _Cellulomonas fimi_ from overnight cultures made from plate 37. (24/07/08)
  • Plates 51/52:* L19 Transforations (rbs\-_dxs_\-Edinbrick) (26/07/08: CF)
  • Plates 53/54:* L20 Transforations (rbs\-_appY_\-Edinbrick) (26/07/08: CF)
  • Plates 55/56:* L21 Transforations (rbs\-_crtB_\-Edinbrick) (26/07/08: CF)
  • Plates 57/58:* L22 Transforations (rbs\-_crtI_\-Edinbrick) (26/07/08: CF)
  • Plates 59/60:* L23 Transforations (PzntA-Edinbrick) (26/07/08: CF)
  • Plates 61/62:* L24 Transforations (_crtE_\-pSB1A2 - from BABEL) (26/07/08: CF)
  • Plates 63/64:* Repeat transformations of L20 (28/07/08: Yan, OG)
  • Plates 65/66:* Repeat transformations of L21 (28/07/08: Yan, OG)
  • Plates 67/68:* glgc mutant 1&2 transformation (29.07.2008: Yan)
  • Plates 69/70:* rbs+dxs, 100 and 900 µl (30.07.2008: CF)
  • Plates 71/72:* crtE coding sequence in Babel2, 100 and 900 µl (30.07.2008: CF)
  • Plate 73:* Subs from Plate 67 (30.07.2008: CF)
  • Plate 74:* Subs from Plates 69-72 (30.07.2008: CF)
  • Plates 75/76:* L26 (Edi-rbs\-_appY_) 100 microlitres/900 microlitres (31.07.2008: HX)
  • Plates 77/78:* L27 (Edi-rbs\-_crtB_) 100 microlitres/900 microlitres (31.07.2008: HX)
  • Plates 79/80:* L28 (Edi\-_cenA_) 100 microlitres/900 microlitres (31.07.2008: HX)
  • Plates 81/82:* L29 (Edi\-_cex_) 100 microlitres/900 microlitres (31.07.2008: HX)
  • Plate 83:* Subs from Plate 76 (L26: Edi-rbs+appY) (01.08.2008: OG)
  • Plate 84:* Subs from Plate 77 (L27: Edi-rbs+crtB) (01.08.2008: HX)
  • Plate 85:* Subs from Plate 80 (L28: Edi+cenA) (01.08.2008: AM)
  • Plate 86:* Subs from Plates 81/82 (L29: Edi+cex) (01.08.2008: AM)
  • Plate 89/90:* L30 (Self-ligation of P15) 100ml/900ml (06.08.2008: HX)
  • Plate 91/92:* L31 (Double digestion of P36/P39 by OG) 100ml/900ml (06.08.2008: HX)
  • Plate 93:* Subculture from Plate 56 (M67, pSB1A2-rbs\-_crtB_) (05.08.08: CF)
  • Plate 94:* Subculture from Plate 89,90 (07.08.08: HX)
  • Plate 95:* Subculture from Plate 92 (07.08.08: HX)
  • Plate 96:* LB, ampicillin plate spread with subcultures from plate 43. (HX, 07.08.08)
  • Plate 97:* LB, ampicillin, 2% glucose plate spread with subcultures from plate 43. (HX, 07.08.08)
  • Plates 98/99:* Transformation of L32 (_crtB_/_crtI_) (08.08.08: HX)
  • Plate 100/101:* Repeat transformation of L31 (Double digestion of P36/P39 by OG) (08.08.08: HX)
  • Plate 102:* Patches from plate 99 (L32, crtBI) (10.08.08: CF)
  • Plate 103:* Patches from plate 101 (L31, glgC double mutant+pSB1A2) (10.08.08: CF)
  • Plate 104/105:* Transform L36 pSB1A2-crtY 100µl/900µl (10.08.08: CF)
  • Plate 106/107:* Transform L37 pSB1A2-rbs-crtY 100µl/900µl (10.08.08: CF)
  • Plate 108:* Subcultures from plates 105 and 106 (11.08.08: CF)
  • Plate 109/110:* Transformation of L33 pSB1A2-PcstA 100µl/900µl (11.08.08: Yan)
  • Plate 111/112:* Transformation of L34 dxs-crtE 100µl/900µl (11.08.08: Yan)
  • Plate 113/114:* Transformation of L35 dxs-lims 100µl/900µl (11.08.08: Yan)
  • Plate 115:* Subs from L33 (Pl. 109/110: pSB1A2-PcstA), L34 (Pl. 111/112: dxs-crtE) and L35 (Pl. 113/114: dxs-lims)
  • Plates 116/117:* Transformation of L38 (psb1A2\-_crtE_) (13.08.08: HX)
  • Plate 118:* Subs from plate 116 (pSB1A2+crtE 100 microlitres). (14.08.08: AH)
  • Plate 119/120:* Transformation of L40 (CenA+Edinbrick1) (14.08.08: YAN)
  • Plate 121/122:* Transformation of L39 (Cex+Edinbrick1) (14.08.08: YAN)
  • Plate 123/124:* Repeat to transform with L39 (cex+Edinbrick1)  (15.08.08: HX)
  • Plate 125/126:* Transform with L40 (cenA+Edinbrick1) (15.08.08: HX)
  • Plate 127/128:* Transform with L41 (dxs/crtE) (15.08.08: HX)
  • Plate 129:* CenA & CeX white colonies from Plates 120 & 123-126 (16/08: CF)
  • Plate 130:* dxs-CrtE white colonies from plate 127-128 (16/08: CF)
  • Plate 131/132:* maize pENTR221 transformation, ISA1 Kan 10 (16/08: CF)
  • Plate 133/134:* maize pENTR221 transformation, ISA2 Kan 10 (16/08: CF)
  • Plate 135:* patches(colonies) from plates 131,133 (16/08: CF)
  • Plate 136/137:* Transformation with L48 (dxs+Lims+Appy) (20.08.08: Yan)
  • Plate 138/139:* Transformation with L49 (CrtBI+Appy) (20.08.08: YAN)
  • Plate 140/141:* Transformation with Edinbrick1 only as control (20.08.08: YAN)
Retrieved from "http://2008.igem.org/Edinburgh/Notebook/Plates"