"
Team:Hawaii/Notebook/2008-08-23
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants (cont.)
- Grace
- Plasmid prep of nir+rbs, rbs+GFP
| Plasmid prep | DNA concentration | 260/280 | 260/230 |
|---|---|---|---|
| nir+rbs | 394.63 ng/μl | 2.04 | 2.36 |
| rbs+GFP | 94.9 ng/μl | 2.06 | 2.17 |
3A assembly
- Grace
File:082308REdigests.jpg
EtBr stained 2% agarose gel ran at 60V for 3 hours. Thirty microliters of RE digest reaction were loaded into each well.
- Gel purified RE digests
- Ligated with pSB1A2:
- nir+rbs
- plac+rbs
- rbs+GFP
- GFP+tt
- GFPf+tt
- Transformed DB3.1 cells with 5μl ligation reaction
BB-pRL1383a MCS re-replacement
- Grace
- Gel purified RE digests
- Ligated J33207 with BB-pRL1383a
- Transformed DH5&alpha cells with 5 μl ligation reaction
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
