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Things we did today

Wetlab work

Construction of p+r

EtBr stained 2% agarose gel ran at 60V for 2.25 hours. Thirty microliters of RE digested DNA were loaded into each well.

Grace

• Ran RE digested products on gel
• Extracted nir, C0012 vector, BB-pRL1383a from gel
• 3A assembly (ligation) of C0012 vector (pSB1A2) with:

• nir and rbs (B0034)
• plac and rbs(B0034)
• pSB1A2 (negative control)

• Transformed DB3.1 cells using 5 μl ligation reaction

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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