Team:Chiba/Calendar-Home/31 August 2008

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30 August 2008 <|> 1 September 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

Transformation

Competent Cells : XL10G

[http://partsregistry.org/Part:BBa_K084007 BBa_K084007(Plac+RBS+LasI)]
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008(Plac+RBS+RhlI)]

---> We saved these plates with a refrigerator.

Digestion

[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

Sample No.	1~3	4
Sample DNA	12	10
PstⅠ	0.2	0.2
XbaⅠ	0.2	-
SpeⅠ	-	0.4
Buffer 2	-	1.5
Buffer 3	2	-
BSA	2	1.5
dH₂O	3.6	1.4
TOTAL	20	15

--->(1/9)Gel Check

(30/8)--->Mini prep

--->Digestion test

Plac+RBS+RhlI Sample No.2~5
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②

Sample	Single Digesiton 2~5	Double Digestion 2~5	Single Digestion T9002①	Single Digesiton T9002②	Double Digestion T9002①②
Sample DNA	1	3	3	1	3
XbaⅠ	0.1	0.1	0.1	0.1	0.1
SpeⅠ	-	0.1	-	-	0.1
Buffer 2	0.9	0.8	0.9	0.9	0.8
BSA	1	1	1	1	1
dH₂O	7	5	5	7	5
TOTAL	10	10	10	10	10

--->Gel Check

Sample No.	Plac+RBS+RhlI Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①②	Single & Double Digestion
Sample DNA	1	10
Loading Dye	1	2
dH₂O	4	-
TOTAL	6	12

From Left

Single Digestion Plac+RBS+RhlI 2~5
Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
Double Digestion Plac+RBS+RhlI 2~5
Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②

Team:Output

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
PCR product([http://partsregistry.org/Part:BBa_J52008 BBa_J52008])②

Sample No.	①	②
DNA	10	10
SpeⅠ	1	0
PstⅠ	1	1
XbaⅠ	0	1
BSA(x100)	0.5	0.5
Buffer2	5	0
Buffer3	0	5
dH₂O	28.5	28.5
TOTAL	50	50

-->37℃ 3hour

-->①20μl refine

-->②20μl refine

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