Team:Warsaw/Calendar-Main/1 July 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. PCR on pB30D plasmid with OmpaL_N and OmpaP_link primers (15 cycles, elongation duration 45 s, elongation temperature 63°C)
  2. PCR on pUC19 plasmid with AlphaL_link and AlphaP_XB primers (20 cycles, elongation duration 45 s, elongation temperature 63°C)
  3. PCR on pUC19 plasmid with OmegaL_link and OmegaP_EPB primers (20 cycles, elongation duration 30 s, elongation temperature 58°C)
    As a result we got three PCR products: OmpA_linker and two fragments of TEM1 beta-lactamese: linker_alpha and linker_omega.
  4. Gel electrophoresis of PCR products and gel-out of proper bands