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Judging/Variance/LCG-UNAM Mexico
From 2008.igem.org
Request
Dear iGEM judges,
The team LCG-UNAM-MEXICO is participating this year for the first time, and I'm writing on behalf of the team to request permission for submitting our Biobricks in non-standard vectors.
Our project requires very tight regulation of E. coli nickel extrusion pump (RcnA) through several regulatory upstream proteins. Our Analysis suggest that we need to control very accurately the production of several molecules and a crucial part of this requires stable low copy-number vectors. For this reason we would like to use pRK415 and pBBIMCS-5 derivatives as our plasmid backbones. We will be adding Biobrick prefix and suffix to both of them. We will also make sure that our parts are easily extracted from the plasmid either through digestion with commercially available enzymes or through PCR.
All this information will be shared to ensure that our Biobricks are useful for the community.
Thanks for your time and consideration
Sur Herrera Paredes
LCG-UNAM-MEXICO Licenciatura en Ciencias Genómicas Universidad Nacional Autónoma de México
Response
Dear Sur:
We need some additional information about this request. It appears you are developing some new BioBrick alpha compatible vectors, which could be very useful in and of themselves (i.e., new parts). We like this idea, but do expect complete documentation about these vectors so they can be used by others in the future.
We are less certain about your proposal to use these vectors to submit your parts to the registry. Having not seen complete documentation of these vectors, we are concerned that there may be difficulties using them to distribute your parts. While low-copy number vectors are useful in many applications, they generally do not facilitate the registry distribution functions.
Sincerely, iGEM judging
