Team:Illinois/Antibody GPCR Fusion Notebook

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== 22nd July ==
  • Yeast obtained from Dr. Zhao


== 24th July ==
  • Prepared liquid culture for DNA extraction
  • Made 1M Tris. Cl pH 8.0
  • Made 4M ammonium acetate


== 22nd August ==
  • Attempted DNA extraction
    • Result: Failed
  • Obtained more yeast from Dr. Zhao


== 25th August ==
  • Prepared overnight culture for DNA extraction (3:27pm)


== 26th August ==
  • Attempted DNA extraction
  • Prepped overnight culture


== 27th August ==
  • Performed PCR (FILL IN PCR TABLE)
  • Prepped 3 overnight cultures


== 28th August == 
  • Extracted DNA from 4 cultures
  • Ran gel of PCR products (1.5%, 200V)
    • Result: No bands present