From 2008.igem.org

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Introduction to Synthetic Biology 2008

Hot (and sexy) Yeast Have you ever forgotten to keep your culture on the stove? Isn´t it annoying to lose several week's work? Tired of apologizing to your boss? WE HAVE THE SOLUTION!!! Valencia iGEM '08 Team heat it up in your lab!!! Besetes en el culete! --- No asustarse, estamos probando colores ---

Main Project - Infector Detector Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)

Main Achievements Designed and modelled a device to detect biofilms and prevent urinary catheter infections like MRSA Successfully utilized cell-free chassis for Infector Detector Characterised Infector Detector to detect AHL at ambient temperatures Compared system expression to AHL Receiver (BBa_F2620) transfer function.

• Discount DNA Synthesis from Geneart. iGEM's DNA synthesis partner offers iGEM teams highly discounted DNA synthesis: $0.20 per base and $35 shipping. Check out the resources box for more information.

• Are you having trouble transforming your DNA from the 2008 Spring DNA Distribution? Please stay tuned. We have sent out a hard copy of the first volume of out iGEM Newsletter which has information discussing this with tips on how to get better results. Download the online pdf version of the newsletter here.

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iGEM Chiba 2008 Team Wiki

HOME | PROJECTS | MEMBERS | CONTACT | INTERNAL

Welcome! Esto va dentro de la tabla welcome???

Members Takayoshi Kubo Yoshimi Iyama Kohei Kawasaki Takahiro Katori Masahiro Tominaga Hiroki Fukutomi Mai Sugiyama Aoi Kobayashi

Specification	Design Solution	System Level
Health & Safety - System must not contain living cells, or cause any harm to patient.	Use a Cell Free System e.g. Promega's S30 Cell Extract	Chassis
Shelf-life - System must have a shelf life of 7 days.	Use a Cell Free System that can be packaged and stored in a -80°C freezer for 7 days.	Chassis
Packaging and Application - System must be applied as a cream or spray.	Package the system in liquid form.	Chassis
Inputs - System must be sensitive to AHL concentration between 5-50nM.	Sensitive AHL receiver to detect low AHL levels - Part no. F2620.	Construct
Outputs - System must give a visual signal if AHL is present.	Couple AHL to a reporter system expressing fluoresent protein eg. RFP	Construct
Operating Conditions - System must operate within temperature 20°-30°C.	Gene expression systems and protein must be thermostable. Characterise a Cell Free System at these temperatures.	Construct & Chassis
Response Time - System needs to have a response time under 3 hours.	To be Determined - this is hard to design for. Protease Inhibitor of Cell Extract should ensure degradation of Visual Reporter is minimal.	Construct & Chassis

Properties	Rabbit Reticulocyte Lysate	Wheat Germ Extract	E. coli Extract	Reconstituted Extract
Types	Eukaryotic	Eukaryotic	Prokaryotic	Artificial
Uses	Widely used for in-vitro translation	Mostly used for in-vitro translation	Mostly used for coupled transcription-translation	Used for in-vitro translation
Templates	mRNAs from viruses or eukaryotes Capped or un-capped mRNAs	mRNAs from viruses or eukaryotes Capped mRNAs	Viral mRNAs with stable secondary structures Un-capped mRNAs Plasmid or linear DNAs	Capped or uncapped mRNAs
Post-translational modifications	Yes	Yes	No	Yes

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