Team:Warsaw/Calendar-Main/11 June 2008

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Blue/white and rifampicin test

Michał L., Ewa, Marcin:

  1. Transformation of E. coli TOP10 carrying pZC320 with:
    • pMPMT5,
    • pMPMT5-AID,
    • pMPMT5-AIDT7,
    • pMPMT5-AID+T7.
  2. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
  3. Rifampicin test of transformants.

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