Arabinose was added to mid log phase cultures of pVJ4 (containing the Cell Lysis Cassette and an Arabinose inducible promoter) and pDKL02 (Cell Lysis Cassette and IPTG inducible promoter) and left overnight.
Results:
pVJ4 + Arabniose - completely clear solution
pVJ4 + IPTG - lots of growth
pVJ4 alone - lots of growth
pDKL02 + Arabinose - growth
pDKL02 + IPTG - growth
These results indicate that the pDKL02 promoter is rather leaky and not as tight as the Arabinose induced promoter. The clear solution of pVJ4 and Arabinose was placed in more LB to see if growth would occur. There was no further growth signifying that cell lysis had occurred. The R gene of the cassette dissolves the membrane when expressed hence the clearing of the solution.
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