"
Team:Heidelberg/Notebook/Sensing Group/Notebook/1stweek
From 2008.igem.org
Revision as of 09:44, 26 October 2008 by Koljaschleich (Talk | contribs)
Contents |
Monday, 08/04/2008
Preparations
Preparations were done at the end of the week before
- Transformation of pDK48 and pTrc99a in E. coli DH5a (0.5 µl plasmid-DNA + 50 µl competent cells)
- Glycerol-stock of Vibrio harveyi BB120, BB886, mm30, BB178, BB125
- picked pTrc99a and pDK48 cultures and inoculated in 3 ml LB overnight
- V. harveyi cultured on LB+-Agar plate
- MiniPrep of pTrc99a and pDK48 from DHha
Cloning of LuxP
- PCR of LuxP from V. harveyi colony
