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Team:Heidelberg/Notebook/Sensing Group/Notebook/3rdweek
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Contents |
Monday, 08/18/2008
- PCR for LuxQ-1 and LuxQ-2 for Fusion constructs. Different Mg concentrations between 1-4 % were used. For LuxQ-2 another PCR was run using as template V. harveyi colonies
- Fusion-PCR for Fusion-1 and Fusion-2, each 4 tubes (2 µl Tar, 1 µl LuxQ)
- preparation of O/N culture of LuxS and pDK48 transformed cells for subsequent miniprep
- digestion of pDK48 with NcoI/NdeI
- digestion of pTrc99alpha and LuxQ (with BamHI and NcoI) and gelextraction
