Team:Princeton/Experiments

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PRINCETON IGEM 2008

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Lab Protocols

1. Primer Design

2. PCR

3. Run Gel

4. Gel Extraction

5. Digestion

6. CIP Treatment

7. PCR Purification

8. Ligation

9. Transformation and Plating

10. Extract DNA (Miniprep)

11. Restriction Map/ Digest

12. Re-transform with selected plasmid

13. Extract DNA (Maxiprep or Midiprep)

14. Sequence

Cell Culture