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Team:Princeton/Experiments
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PRINCETON IGEM 2008
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Bio-brick Creation
Lab Protocols
1. Primer Design
2. PCR Amplification
3. PCR SOEing
4. Run Gel
5. Gel Extraction
6. Digestion
8. CIP Treatment
8. PCR Purification
9. Ligation
10. Transformation and Plating
11. Extract DNA (Miniprep)
12. Restriction Map/ Digest
13. Re-transform with selected plasmid
14. Extract DNA (Maxiprep or Midiprep)
15. Sequence
Bio-Brick Verification
Cell Culture Process
1. Lentivirus Production
2. Lentivirus Harvesting
3. Infection
Also,
1. Transfection (nonviral)
