Team:Princeton/Experiments

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PRINCETON IGEM 2008

Home	Project Overview	Project Details	Experiments	Results	Notebook

Parts Submitted to the Registry	Modeling	The Team	Gallery

Lab Protocols

1. Primer Design

2. PCR Amplification

3. PCR SOEing

4. Run Gel

5. Gel Extraction

6. Digestion

8. CIP Treatment

8. PCR Purification

9. Ligation

10. Transformation and Plating

11. Extract DNA (Miniprep)

12. Restriction Map/ Digest

13. Re-transform with selected plasmid

14. Extract DNA (Maxiprep or Midiprep)

15. Sequence

Cell Culture Process

1. Lentivirus Production

2. Lentivirus Harvesting

3. Infection

Also,

1. Transfection (nonviral)